Cell potential measuring electrode and measuring apparatus using the same
First Claim
1. A cell potential measuring electrode assembly suitable for measuring electrical potential in a neural sample comprising:
- a. a plurality of measurement microelectrodes insulated from each other and located on an insulating substrate in a measuring region,b. a plurality of reference electrodes located on said insulating substrate and isolated from each other in said measuring region, each of said plurality of reference electrodes having an impedance smaller than said each of said measurement electrodes when measured in an electrolyte covering said measuring region at 1 kHz, 50 mv.
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Abstract
This invention relates to a low impedance cell potential measuring electrode assembly typically having a number of microelectrodes on an insulating substrate and having a wall enclosing the region including the microelectrodes. The device is capable of measuring electrophysiological activities of a monitored sample using the microelectrodes while cultivating those cells or tissues in the in the region of the microelectrodes. The invention utilizes independent reference electrodes to lower the impedance of the overall system and to therefore lower the noise often inherent in the measured data. Optimally the microelectrodes are enclosed by a physical wall used for controlling the atmosphere around the monitored sample.
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Citations
24 Claims
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1. A cell potential measuring electrode assembly suitable for measuring electrical potential in a neural sample comprising:
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a. a plurality of measurement microelectrodes insulated from each other and located on an insulating substrate in a measuring region, b. a plurality of reference electrodes located on said insulating substrate and isolated from each other in said measuring region, each of said plurality of reference electrodes having an impedance smaller than said each of said measurement electrodes when measured in an electrolyte covering said measuring region at 1 kHz, 50 mv. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A cell potential measuring electrode comprising plural microelectrodes disposed on an insulating substrate, a conductive pattern for wiring of said microelectrodes, an electric contact connected to the end of said conductive pattern, an insulating film covering the surface of said conductive pattern, and a wall enclosing the region including the microelectrodes on the surface of said insulating film, being a cell potential measuring electrode for use in measurement of electrophysiological activities while cultivating cells or tissues in a region enclosed by said wall,
wherein reference electrodes having a smaller impedance than the impedance of said microelectrodes are respectively disposed at plural positions located on said insulating substrate in the region enclosed by said wall and at a specific distance from the region of disposition of said microelectrodes, electric contacts are further connected between the conductive pattern for wiring of each reference electrode and the end of said conductive pattern, and the surface of the conductive pattern for wiring of said reference electrodes is covered with said insulating film.
Specification