Liver regeneration using pancreas cells

• US 6,132,708 A
• Filed: 10/10/1997
• Issued: 10/17/2000
• Est. Priority Date: 10/10/1997
• Status: Expired due to Fees

• Current Assignee: Oregon Health & Science University
• Original Assignee: Oregon Health & Science University
• Inventors: Grompe, Markus
• Primary Examiner(s): Clark, Deborah J.
• Application Number:
  US08/949,067
• Time in Patent Office: 1,103 Days
• Field of Search: 424/93.1, 424/43.2, 424/93.21, 424/93.7, 435/325
• US Class Current: 424/93.1
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61K 35/39 Pancreas; Islets of Langerhans Langerhans cells of epidermis A61K35/36
• CPC Class Codes: C12N 5/0676 Pancreatic cells

Abstract

This invention provides a method of regenerating a functional liver by transplantation of pancreas cells. Also provided are pancreas cell capable of regenerating functional liver tissue.

Claims

1. A method of regenerating liver function in an individual, the method comprising

(a) introducing an effective amount of pancreas cells into the spleen of the individual, wherein said pancreas cells are derived from the same species as the individual;

and

(b) allowing the pancreas cells to regenerate at least one liver function selected from the group consisting of interconversion of metabolites, blood protein manufacture, erythropoietic function, detoxification, bile formation and vitamin storage.

2. The method according to claim 1 wherein the individual is a mammal.

3. The method according to claim 2 wherein the mammal is a human.

4. The method according to claim 1 wherein the pancreas cells are an autograft.

5. The method according to claim 1 wherein the pancreas cells are obtained from an in vitro culture.

6. The method of claim 1, wherein between about 10³ to about 10¹² pancreas cells are introduced into the spleen of the individual.

7. The method of claim 6, wherein between about 10⁶ to about 10⁹ pancreas cells are introduced into the spleen of the individual.

8. The method of claim 1, wherein said introducing is accomplished by injecting said pancreas cells into the spleen of the individual.

9. A method of ameliorating symptoms of a liver disease in an individual, the method comprising:

introducing pancreas cells into the spleen of the individual, wherein the pancreas cells are derived from the same species as the individual and are capable of regenerating at least one liver function selected from the group consisting of interconversion of metabolites, blood protein manufacture, erythropoietic function, detoxification, bile formation and vitamin storage; and

ameliorating symptoms of said disease, wherein said amelioration is effected by the regeneration of said at least one liver function.

10. The method according to claim 9 wherein the individual is a human.

11. The method according to claim 9 wherein the pancreas cells are an autograft.

12. The method according to claim 9 wherein the pancreas cells are obtained from an in vitro culture.

13. The method of claim 9, wherein between about 10³ to about 10¹² pancreas cells are introduced into the spleen of the individual.

14. The method of claim 9, wherein between about 10⁶ to about 10⁹ pancreas cells are introduced into the spleen of the individual.

15. The method of claim 9, wherein said introducing is accomplished by injecting said pancreas cells into the spleen of the individual.

16. The method of claim 9, wherein the liver disease is selected from the group consisting of hereditary tyrosinemia type I (HT1), hepatitis, cirrhosis, and cancer.