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Method for linear mRNA amplification

  • US 6,132,997 A
  • Filed: 05/28/1999
  • Issued: 10/17/2000
  • Est. Priority Date: 05/28/1999
  • Status: Expired due to Term
First Claim
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1. A method for producing linearly amplified amounts of antisense RNA from mRNA, said method comprising:

  • (a) converting mRNA to double-stranded cDNA, wherein one terminus of said double-stranded cDNA comprises an RNA polymerase promoter region; and

    (b) transcribing said double-stranded cDNA with an RNA polymerase and ribonucleotides into antisense RNA in the presence of a reverse transcriptase that is incapable of RNA-dependent DNA polymerase activity during said transcribing step.

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