Bioreactor system and method for probing toxic materials

• US 6,133,021 A
• Filed: 12/15/1998
• Issued: 10/17/2000
• Est. Priority Date: 02/09/1998
• Status: Expired due to Term

• Current Assignee: Kwangju Institute Of Science And Technology
• Original Assignee: Kwangju Institute Of Science And Technology
• Inventors: Kim, Joong Hyun, Gu, Man Bock
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/212,740
• Time in Patent Office: 672 Days
• Field of Search: 422/82.05, 422/82.06, 422/82.09, 435/287.1, 435/288.7, 435/808, 435/849
• US Class Current: 435/288.7
• CPC Class Codes: C12M 27/02 Stirrer or mobile mixing elements
• CPC Class Codes: C12M 41/22 in contact with the bioreactor walls
• CPC Class Codes: C12M 41/46 of cellular or enzymatic activity or functionality, e.g. cell viability
• CPC Class Codes: G01N 33/1866 using microorganisms G01N33/1806 takes precedence
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/849 Escherichia coli

Abstract

A bioreactor system of probing toxic materials using microorganisms comprises two reactors. A first-step reactor serves as a microorganism reservoir in which the microorganisms are continuously cultured in a constant phase and a second-step reactor, provided with fiber optics, offers a place in which the microorganisms meet the toxic materials for the first time. Since the microorganisms are so genetically recombinant as to luminesce upon reaction with toxic materials, light is generated in the second-step reactor and, then, transmitted along the fiber optics to a luminometer where the change in the intensity of the light is monitored over time. The microorganisms can be stably provided from the reservoir, so that it is possible to continuously monitor the light intensity and thus, to trace the pollution of the toxic materials. The toxic materials may come from any of the sources including rivers, waste water, sewage, agricultural and industrial water, household water, tap water, atomic power plants, etc.

Claims

1. A bioreactor system for detecting toxic materials comprising:

a first-step reactor wherein microorganisms are continuously cultured in a constant phase, said microorganisms being genetically altered so as to emit light upon contact with at least one of a plurality of toxic materials;

a second-step reactor wherein said microorganisms first contact said at least one of said plurality of toxic materials; and

an optical detector optically connected to said second-step reactor;

whereby, said microorganisms emit light which is transmitted to said optical detector.

2. The bioreactor system as set forth in claim 1, wherein said first-step reactor and said second-step reactor each have an operational volume of 57 ml or less.

3. The bioreactor system as set forth in claim 1, wherein said second-step reactor includes:

a reactor tank;

a water jacket surrounding said reactor tank having;

a warm water inlet; and

a warm water outlet;

a magnetic stirring plate; and

a media inlet and a media outlet whereby a nutrient media can be fed and drained from said tank;

a drop wherein said microorganism and said toxic materials are continuously added to said tank;

a gas pipe for discharging a gas resulting from a metabolism of said microorganisms;

a condenser for cooling said gas prior to discharge;

an air filter;

an air conduit for introducing filtered air into said second-step reactor;

a magnetic bar; and

a glass window whereby said emitted light from the microorganisms is transmitted from said second-step reactor to said optical detector.

4. A bioreactor system as recited in claim 1, wherein said microorganism includes E. coli TV1061.

5. A bioreactor system for detecting toxic materials comprising:

a first-step reactor wherein microorganisms are continuously cultured in a constant phase, said microorganisms being genetically altered so as to emit light upon contact with at least one of a plurality of toxic materials;

a second-step reactor wherein said microorganisms first contact said at least one of said plurality of toxic materials; and

an optical detector optically connected to said second-step reactor;

whereby, said microorganisms emit light which is transmitted to said optical detector;

said second-step reactor includes;

a reactor tank;

a waterjacket surrounding said reactor tank having;

a warm water inlet; and

a warm water outlet;

a magnetic stirring plate; and

a media inlet and a media outlet whereby a nutrient media can be fed and drained from said tank;

a drop wherein said microorganism and said toxic materials are continuously added to said tank;

a gas pipe for discharging a gas resulting from a metabolism of said microorganisms;

a condenser for cooling said gas prior to discharge;

an air filter;

an air conduit for introducing filtered air into said second-step reactor;

a magnetic bar; and

a glass window whereby said emitted light from the microorganisms is transmitted from said second-step reactor to said optical detector.

6. A bioreactor system as recited in claim 5, wherein said microorganism includes E. coli TV1061.

7. A bioreactor system for detecting toxic materials comprising:

a first-step reactor wherein microorganisms are continuously cultured in a constant phase, said microorganisms being genetically altered so as to emit light upon contact with at least one of a plurality of toxic materials;

a second-step reactor wherein said microorganisms first contact said at least one of said plurality of toxic materials; and

an optical detector optically connected to said second-step reactor;

whereby, said microorganisms emit light which is transmitted to said optical detector;

said second-step reactor includes;

a media inlet and a media outlet whereby a nutrient media can be fed and drained from said tank;

a drop wherein said microorganism and said toxic materials are continuously added to said tank; and

a glass window whereby said emitted light from the microorganisms is transmitted from said second-step reactor to said optical detector.

8. A bioreactor system as recited in claim 7, wherein said microorganism includes E. coli TV1061.