Platinum-containing compounds methods for their preparation and applications thereof

• US 6,133,038 A
• Filed: 08/10/1998
• Issued: 10/17/2000
• Est. Priority Date: 07/19/1990
• Status: Expired due to Term

• Current Assignee: Kreatech Diagnostics
• Original Assignee: Kreatech Diagnostics
• Inventors: Van Es, Remco Maria, Bloemink, Marieke Johanna, van den Berg, Franciscus Michiel, Lempers, Edwin Leo Mario, Houthoff, Hendrik J., Jelsma, Tinka, Reedijk, Jan
• Primary Examiner(s): Elliott, George C.
• Assistant Examiner(s): SANDALS, WILLIAM O
• Application Number:
  US09/131,625
• Time in Patent Office: 799 Days
• Field of Search: 436/501, 436/84, 435/975, 556/136, 536/24.3
• US Class Current: 436/84
• CPC Class Codes: C07F 15/0093 without a metal-carbon linkage
• CPC Class Codes: C07J 51/00 Normal steroids with unmodified cyclopenta(a)hydrophenanthrene skeleton not provided for in groups C07J1/00 - C07J43/00
• CPC Class Codes: C09B 11/04 derived from triarylmethanes , i.e. central C-atom is substituted by amino, cyano, alkyl
• CPC Class Codes: C12Q 1/6816 characterised by the detection means C12Q1/6804 takes precedence
• CPC Class Codes: C12Q 2563/137 Metal/ion, e.g. metal label

Abstract

The invention provides platinum-based probe compounds having the structure: ##STR1## wherein: Pt is a platinum atom, PROBE is a probe biomolecule for associating to a target biomolecule, M is a detectable marker moiety, and X and Y are stabilizing substituents. Also provided are platinum-based labeling compounds having the structure: ##STR2## wherein: Pt is a platinum atom, M is a detectable marker moiety, A is a displaceable leaving group, and X and Y are stabilizing substituents. The invention further provides platinum-based linker compounds having the structure: ##STR3## wherein: Pt is a platinum atom, A and B are the same or different reactive moieties, and X and Y are stabilizing substituents. Other Pt^II and Pt^IV compounds are also provided. Moreover, the invention provides methods for the preparation and use of these compounds, as well as diagnostic kits which contain the compounds.

Claims

1. A diagnostic kit for detecting biological substances of interest, comprising:

a first container housing a platinum-based linker compound having the formula ##STR35## wherein X represents an aliphatic diamine having 2-6 carbon atoms and wherein A and B represent the same or different reactive moieties, and

a second container housing a detectable marker moiety (M) attachable to said platinum-based linker compound such that the contents of said first and second containers combine to form a platinum-based labeling compound having the formula;

##STR36##

2. The diagnostic kit of claim 1, further comprising at least one additional container housing additional detectable marker moieties attachable to said platinum-based linker compound.

3. The diagnostic kit of claim 1 wherein A and B represent NO₃.

4. The diagnostic kit of claim 1 wherein the molar ratio of said platinum-based linker compound to said detectable marker moiety is about one.

5. A method for preparing a modified platinum-based labeling compound having the structure:

wherein X represents an aliphatic diamine having 2-6 carbon atoms, A is a first reactive moiety and M is a detectable marker moiety, said method comprising the steps of;

(a) providing a compound having the structure;

##STR37## wherein B is a second reactive moiety;

(b) replacing B with M; and

(c) isolating said modified platinum-based labeling compound.

6. The method of claim 5, wherein said aliphatic diamine is ethylenediamine.

7. A method for preparing a detectable probe compound having the structure:

##STR38## wherein PROBE is a biomolecule selected from the group consisting of oligonucleotides, polynucleotides, oligopeptides, polypeptides, proteins, glycoproteins and lipoproteins for associating to a target biomolecule, M is a detectable marker moiety, and X and Y are stabilizing substituents, said method comprising the steps of;

(a) providing a detectable labeling compound having the formula;

##STR39## wherein M is a detectable marker moiety, A is a displaceable leaving group, and X and Y are stabilizing substituents;

(b) replacing A with said PROBE; and

(c) isolating said detectable probe compound.