Process and test kit for cocaine detection

• US 6,133,040 A
• Filed: 08/28/1997
• Issued: 10/17/2000
• Est. Priority Date: 12/26/1994
• Status: Expired due to Term

• Current Assignee: Identa Ltd. (Identa Corp.)
• Original Assignee: Identa Ltd. (Identa Corp.)
• Inventors: Glattstein, Baruch
• Primary Examiner(s): ALEXANDER, LYLE
• Application Number:
  US08/860,435
• Time in Patent Office: 1,146 Days
• Field of Search: 436/92, 436/96, 436/901, 436/164, 422/61
• US Class Current: 436/92
• CPC Class Codes: G01N 33/946 CNS-stimulants, e.g. cocaine, amphetamines
• CPC Class Codes: Y10S 436/901 Drugs of abuse, e.g. narcotics, amphetamine
• CPC Class Codes: Y10T 436/141111 Diverse hetero atoms in same or different rings [e.g., alkaloids, opiates, etc.]
• CPC Class Codes: Y10T 436/145555 Hetero-N

Abstract

Test kit and method for discriminating cocaine and its salts from substances that falsely give a positive color reaction with Scott'"'"'s thiocyanate reagent. As all Scott false positive substances have lower pKa values than cocaine, or in their basic from higher pKa values than the cocaine base, they can be discriminated from cocaine by a suitable pH test of the solution. The pH indicator is preferably organic or lipophilic so that it can be dissolved in a solvent immiscible with water. The partitioning of the organic cocaine base or its salt between an organic and water phase of specific pH is used to further distinguish the test substance.

Claims

1. A process for discriminating cocaine and/or its salts from substances that give a false positive result in a Scott test comprising the following steps:

a) reacting a substance with a cobalt thiocyanate reagent or Scott'"'"'s thiocyanate reagent;

b) applying to a substance that gives a positive result in step a) a solution containing a phthalein pH indicator, wherein

(1) when said substance is a cocaine base the solution turns a cocaine-indicative color or does not change color, or

(2) when said substance is a substance giving a false positive result with Scott'"'"'s test reagents the solution turns a color which is distinguishable from said cocaine-indicative color; and

c) adding a few drops of an aqueous buffer solution of pH 7, if there has been no change in the color of the indicator solution of step b), wherein

(1) when cocaine salts are present the resulting solution appears said cocaine-indicative color, or

(2) when substances giving false positive results with Scott'"'"'s test reagents are present, the resulting solution is a color distinguishable from said cocaine indicative-color.

2. The process according to claim 1, wherein the phthalein indicator is lipophilic or organic or both.

3. The process according to claim 1, wherein the phthalein indicator is tetrabromophenolphthalein ethylester or naphtholphthalein.

4. The process according to claim 1, wherein the color reaction of the phthalein indicator is done in two steps:

a) dissolving a small amount of the phthalein indicator in a solvent immiscible with water and adding a few drops of said indicator solution to the test substance so there is a change in color of the solution when an organic free base is present; and

b) adding a few drops of aqueous buffer of pH 7 if there is no change in the color of the solution in step a), and when there is a salt of an organic base, the organic base is partitioned into the organic phase to produce a solution that is either said cocaine-indicative color or a color distinguishable from said cocaine-indicative color.

5. The process according to claim 4, wherein the solvent immiscible in water is chloroform, dichloromethane or 1,2-dichloroethane.

6. The process according to claim 1 for testing trace amounts of cocaine and/or its salts in the range of less than 1 mg.

7. The process according to claim 1 for discriminating cocaine and/or its salts from phencyclidine, dibucaine, butacaine, methapyrilene, dixyrazine and lidocaine.

8. The process according to claim 1 for discriminating cocaine and/or its salts from procaine, tetracaine and methadone.

9. A kit for detection and identification of cocaine and/or its salts and for use in a process according to claim 1, comprising:

a) a cobalt thiocyanate reagent or Scott'"'"'s thiocyanate reagent;

b) a pH indicator solution wherein the indicator is dissolved in a solvent immiscible with water and wherein said solution turns said cocaine-indicative color or does not change color in the presence of cocaine or turns a color distinguishable from said cocaine-indicative color in the presence of substances giving a false positive result in Scott'"'"'s test; and

c) an aqueous buffer of pH 7 for transferring an organic base to the organic phase.