Particle measuring apparatus

• US 6,133,995 A
• Filed: 05/01/1998
• Issued: 10/17/2000
• Est. Priority Date: 05/09/1997
• Status: Expired due to Term

• Current Assignee: Sysmex Corporation
• Original Assignee: Sysmex Corporation
• Inventors: Kubota, Fumio
• Primary Examiner(s): Kim, Robert H.
• Assistant Examiner(s): Smith, Zandra V.
• Application Number:
  US09/070,811
• Time in Patent Office: 900 Days
• Field of Search: 356/72, 356/73, 356/432, 356/39, 356/337, 422/82.05, 436/63
• US Class Current: 356/73
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 2015/0092 Monitoring flocculation or agglomeration
• CPC Class Codes: G01N 2015/012 Red blood cells
• CPC Class Codes: G01N 2015/018 Platelets

Abstract

A particle measuring apparatus includes a characteristic parameter extracting device for extracting a plurality of characteristic parameters from each particle in a sample, and a distribution diagram preparing device for preparing a first distribution diagram on the basis of the extracted characteristic parameters. It further includes a first separating device for separating a cluster including target particles from others on the prepared first distribution diagram. In addition, a discriminating device is included for setting a specified discrimination standard for the separated cluster including the target particles and for judging whether the particles in the cluster are target particles or non-target particles on the basis of the discrimination standard. Finally, a counting device is included for counting the number of the target particles on the basis of a discrimination result of the discriminating device.

Claims

1. A particle measuring apparatus comprising:

characteristic parameter extracting means for extracting a plurality of characteristic parameters from each particle in a sample;

distribution diagram preparing means for preparing at least one first distribution diagram on the basis of the extracted characteristic parameters;

first separating means for separating a cluster including target particles from other information on the prepared at least one distribution diagram;

discriminating means for calculating a discrimination function for the separated cluster including the target particles and for discriminating target particles from non-target particles in the cluster on the basis of a distance from the calculated discrimination function; and

counting means for counting the number of the target particles discriminated by the discriminating means.

2. A particle measuring apparatus according to claim 1, further comprising a second separating means for finely separating the cluster including the target particles into a plurality of clusters on the first distribution diagram, wherein the distribution diagram preparing means prepares a second distribution diagram for each of the finely separated clusters, and the discriminating means calculates a discrimination function for at least one cluster among the plurality of clusters.

3. A particle measuring apparatus according to claim 2, wherein the discrimination function is prepared based upon the second distribution diagram, and the discriminating means discriminates a particle type on the basis of distance from the discrimination function.

4. A particle measuring apparatus according to claim 3, wherein the discriminating means discriminates the particles positioned at least a given distance from the discrimination function as the target particles.

5. A particle measuring apparatus according to claim 3, wherein the discriminating means discriminates the particles positioned at least a given distance from the discrimination function as the non-target particles.

6. A particle measuring apparatus according to claim 1, further comprising an image capturing section, an image capture controlling section and an image processing section, wherein the image capture controlling section operates the image capturing section for each particle in a cluster including the target particles on the basis of the extracted characteristic parameters of the particles, the image processing section processes the image obtained by the image capturing section to detect a presence or absence of a particle in the image, and the discriminating means discriminates whether the particle is a target particle or a non-target particle based on a presence or absence detected in the image processing section.

7. A particle measuring apparatus according to claim 4, wherein the target particles are aggregations of platelets.

8. A particle measuring apparatus according to claim 5, wherein the target particles are platelets and the non-target particles are background noise.

9. A particle measuring apparatus according to claim 1, wherein the characteristic parameter extracting means comprises a sheath flow cell for forming a fine sample stream in which a sample liquid containing the particles is surrounded by a sheath liquid, a light applying means for applying light to the sample liquid made into the fine stream, a detecting means for detecting the light scattered by the particles to output electric signals, and a calculating means for calculating the characteristic parameters of the particles from the detected light.

10. A particle measuring apparatus according to claim 1, further comprising a second separating means for finely separating the cluster including the target particles into a plurality of clusters on the first distribution diagram, the discriminating means discriminating one of the plurality of clusters as the non-target particles.

11. A particle measuring apparatus according to claim 10, wherein the first separating means separates a first area containing platelets as the target particles from other information on the first distribution diagram, the second separating means separates a second area containing fragmented erythrocytes from other information within the first area, and the counting means counts the number of particles contained in the first areas, excluding the second area, as the number of platelets.

12. A particle measuring apparatus according to claim 11, wherein the second separating means is comprised of a distribution operating means for obtaining a frequency distribution of the cluster in the first area containing platelets on the prepared distribution diagram, and a first separating line drawing means for setting up a first separating line for separating the second area containing fragmented erythrocytes on a statistical parameter of the frequency distribution.

13. A particle measuring apparatus according to claim 11, further comprising a third separating means for separating, within the first area, a third area containing fragmented leukocytes from other information, and a second counting means for excluding the number of the particles present in the third area from the number of the particles counted by the first counting means.

14. A particle measuring apparatus according to claim 13, further comprising a fourth separating means for separating, within the first area, a fourth area containing reticulated platelets from other information, and a third counting means for counting only the number of the particles present in the fourth area from the number of the particles counted by the second counting means.

15. A particle measuring apparatus according to claim 14, wherein the fourth separating means comprises a second separating line drawing means for setting up a second separating line for separating the fourth area containing reticulated platelets on the basis of the statistical parameter of the frequency distribution.

16. A particle measuring apparatus according to claim 1, wherein the discrimination function is an estimation curve.

17. A particle measuring apparatus according to claim 16, further comprising a second separating means for finely separating the cluster including the target particles into a plurality of clusters on the first distribution diagram, wherein the distribution diagram preparing means prepares a second distribution diagram for each of the finely separated clusters, and the discriminating means calculates the estimation curve for at least one cluster among the plurality of clusters.

18. A particle measuring apparatus according to claim 17, wherein the estimation curve is prepared based upon the second distribution diagram, and the discriminating means discriminates a particle type on the basis of distance from the estimation curve.

19. A particle measuring apparatus according to claim 18, wherein the discriminating means discriminates the particles positioned at least a given distance from the estimation curve as the target particles.

20. A particle measuring apparatus according to claim 18, wherein the discriminating means discriminates the particles positioned at least a given distance from the estimation curve as the non-target particles.

21. A particle measuring apparatus according to claim 1, wherein the target particles are aggregations of platelets.

22. A particle measuring apparatus comprising:

characteristic parameter extracting means for extracting a plurality of characteristic parameters from each particle in a sample;

distribution diagram preparing means for preparing at least one distribution diagram on the basis of the extracted characteristic parameters;

first separating means for separating a cluster including target particles from other information on the prepared at least one distribution diagram;

discriminating means for setting a specified discrimination standard for the separated cluster including the target particles and for discriminating target particles from non-target particles in the cluster on the basis of the discrimination standard; and

counting means for counting the number of the target particles discriminated by the discriminating means, wherein the target particles are platelets and the non-target particles are background noise.

23. The particle measuring apparatus of claim 22, wherein the distribution diagram preparing means prepares a second distribution diagram on the basis of the separated cluster, and the discrimination means sets the specified discrimination standard as a function on the second distribution diagram.

24. The particle measuring apparatus of claim 23, wherein the discrimination means discriminates the platelets from the background noise based upon distance from the function.

25. The particle measuring apparatus of claim 23, wherein the function is an estimation curve.

26. The particle measuring apparatus of claim 24, wherein the function is an estimation curve.

27. The particle measuring apparatus of claim 23, wherein the discriminating means discriminates the particles positioned at least a given distance from the function as background noise.

28. The particle measuring apparatus of claim 24, wherein the discriminating means discriminates the particles positioned at least a given distance from the function as background noise.

29. The particle measuring apparatus of claim 25, wherein the discriminating means discriminates the particles positioned at least a given distance from the function as background noise.

30. The particle measuring apparatus of claim 26, wherein the discriminating means discriminates the particles positioned at least a given distance from the function as background noise.

31. A particle measuring apparatus comprising:

characteristic parameter extracting means for extracting a plurality of characteristic parameters from each particle in a sample;

distribution diagram preparing means for preparing a first distribution diagram on the basis of the extracted characteristic parameters;

first separating means for separating a cluster including target particles from other information on the prepared first distribution diagram;

second separating means for finely separating the cluster including target particles into a plurality of clusters on the first distribution diagram;

discriminating means for setting a specified discrimination standard for the separated cluster including the target particles and for discriminating target particles from non-target particles in the plurality of clusters on the basis of the discrimination standard and discriminating one of the plurality of clusters as non-target particles; and

counting means for counting the number of the target particles discriminated by the discriminating means.

32. A particle measuring apparatus according to claim 31, wherein the first separating means separates a first area containing platelets as the target particles from other information on the first distribution diagram, the second separating means separates a second area containing fragmented erythrocytes from other information within the first area, and the counting means counts the number of particles contained in the first area, excluding the second area, as the number of platelets.

33. A particle measuring apparatus according to claim 32, wherein the second separating means is comprised of a distribution operating means for obtaining a frequency distribution of the cluster in the first area containing platelets on the prepared distribution diagram, and a first separating line drawing means for setting up a first separating line for separating the second area containing fragmented erythrocytes on a statistical parameter of the frequency distribution.

34. A particle measuring apparatus according to claim 32, further comprising a third separating means for separating, within the first area, a third area containing fragmented leukocytes from other information, and a second counting means for excluding the number of the particles present in the third area from the number of the particles counted by the first counting means.

35. A particle measuring apparatus according to claim 34, further comprising a fourth separating means for separating, within the first area, a fourth area containing reticulated platelets from other information, and a third counting means for counting only the number of the particles present in the fourth area from the number of the particles counted by the second counting means.

36. A particle measuring apparatus according to claim 35, wherein the fourth separating means comprises a second separating line drawing means for setting up a second separating line for separating the fourth area containing reticulated platelets on the basis of the statistical parameter of the frequency distribution.