Methods of reducing the levels of protoporphyrin IX in recombinant hemoglobin preparations
First Claim
1. A method of reducing protoporphyrin IX (PIX)-containing hemoglobin in the production of recombinantly produced hemoglobin, said method comprising inserting a promoter into a host cell expressing exogenous hemoglobin, wherein said promoter causes an increase in the expression of the hemH gene.
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Abstract
The present invention relates to recombinant hemoglobin solutions containing reduced amounts of protoporphyrin IX and to methods of producing such hemoglobin solutions. The methods are accomplished by overexpressing the hemH gene in a suitable host cell or by increasing the production of hemH in a host cell, for example, by integration of the hemH gene into the chromosome of the host cell or by inserting multiple copies of the gene in host cells naturally having a hemH gene.
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Citations
13 Claims
- 1. A method of reducing protoporphyrin IX (PIX)-containing hemoglobin in the production of recombinantly produced hemoglobin, said method comprising inserting a promoter into a host cell expressing exogenous hemoglobin, wherein said promoter causes an increase in the expression of the hemH gene.
- 4. A method of reducing protoporphyrin IX (PIX)-containing hemoglobin in the production of recombinantly produced hemoglobin, said method comprising inserting multiple copies of the hemH gene into a host cell expressing exogenous hemoglobin, wherein said hemH genes are expressed therein.
Specification