Assay methods and apparatus

• US 6,136,611 A
• Filed: 07/31/1997
• Issued: 10/24/2000
• Est. Priority Date: 07/31/1997
• Status: Expired due to Term

• Current Assignee: Research International Incorporated
• Original Assignee: Research International Incorporated
• Inventors: Jung, Charles C., Saaski, Elric W.
• Primary Examiner(s): Chin, Christopher L.
• Application Number:
  US08/904,421
• Time in Patent Office: 1,181 Days
• Field of Search: 422/55, 422/57, 422/58, 422/82.05, 422/82.08, 422/82.09, 422/82.11, 435/287.1, 435/287.2, 435/808, 436/164, 436/165, 436/172, 436/518, 436/527, 436/525, 436/535, 436/805
• US Class Current: 436/527
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G02B 6/241 Light guide terminations
• CPC Class Codes: G02B 6/262 Optical details of coupling light into, or out of, or between fibre ends, e.g. special fibre end shapes or associated optical elements
• CPC Class Codes: G02B 6/4204 the coupling comprising intermediate optical elements, e.g. lenses, holograms encapsulated active devices H01S5/02208, H01L33/52
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

An optical assay apparatus is described which includes a light source module and an optical sensing element coupled by an interrogation module. The light source module produces light having propagation angles ranging from a lower, non-zero limit. This is accomplished by including an obscuration which blocks low propagation angle light. The sensing element includes a reflector portion and an sensing fiber portion. The reflector portion receives, as incident light, the light produced by the light source module and produces, as reflected light, light having an approximately constant propagation angle, preferably just less than the critical angle of the sensing fiber. The sensing element also includes a lens position which collimates signal recovery light collected by the sensing fiber. The interrogation module includes a window containing a light source optical fiber that transmits light from the light source module to the sensing element. The light source fiber has an angled end with a reflective surface to form a right-angle reflector and is embedded within the window in a slot containing an opaque material for absorbing back-scattered light. The sensing fiber may be appropriately adapted for evanescent-wave or surface plasmon resonance sensing operations.

Claims

1. An optical assay apparatus for detecting analytes in a sample, the apparatus comprising:

a light source module operable to produce light rays directed at a range of angles relative to an optical axis of the assay apparatus;

an optical sensor optically coupled with the light source module and including a ray redirection portion and a sensing fiber portion, the ray redirection portion operable to receive the light rays produced by the light source module and to correspondingly provide light rays directed at an approximately constant angle to the sensing fiber portion to correspondingly produce an evanescent electric field, the sensing fiber portion collecting light emitted from the sample in response thereto; and

an interrogation module optically coupled with the optical sensor and operable to receive the collected light emitted from the sample.

2. The optical assay apparatus of claim 1 wherein the range of angles of the light rays produced by the light source module vary from angles greater than the minimum angle necessary to intercept the ray redirection portion to angles less than or equal to the maximum angle produced by the light source module.

3. The optical assay apparatus of claim 1 wherein the light source module includes an obscuration positioned relative to the optical axis to selectively block light rays directed at angles less than the minimum angle necessary to intercept the ray redirection portion.

4. The optical assay apparatus of claim 1 wherein the ray redirection portion and the sensing fiber portion of the optical sensor are formed together as a single piece.

5. The optical assay apparatus of claim 1 wherein the ray redirection portion of the optical sensor includes a refractive surface operable to collect the emitted light collected by the sensing fiber portion and provide the collected light to the interrogation module.

6. The optical assay apparatus of claim 1 wherein the ray redirection portion of the optical sensor includes a reflective surface operable to receive, as incident light, the light rays produced by the light source module and to provide, as reflected light, the light rays directed at an approximately constant angle to the sensing fiber portion.

7. The optical assay apparatus of claim 6 wherein the ray redirection portion of the optical sensor further includes a refractive surface operable to refract the light rays produced by the light source module.

8. The optical assay apparatus of claim 1 wherein the approximately constant angle is approximately less than a critical angle relative to the optical axis.

9. The optical assay apparatus of claim 1 wherein the optical sensor is coupled with the light source module by the interrogation module.

10. The optical assay apparatus of claim 9 wherein the interrogation module include s a window in which a portion of an optical fiber is embedded, the optical fiber transmitting the light rays produced by the light source module to the optical sensor.

11. The optical assay apparatus of claim 9 wherein the interrogation module includes a window in which a portion of an optical fiber is embedded, the optical fiber being coupled at a first end portion with the light source module to receive the light rays produced thereby, the optical fiber having a reflective surface at a second end portion, the reflective surface reflecting the light rays produced by the light source module to the optical sensor.

12. The optical assay apparatus of claim 9 wherein the interrogation module includes a window having a groove containing light absorbing material with a portion of an optical fiber embedded therein, the optical fiber transmitting the light rays produced by the light source module to the optical sensor.

13. An assay apparatus, comprising:

a light source module operable to produce light having a range of propagation angles; and

a sensor operable to receive the light produced by the light source module, the sensor including a light adjusting portion and an assay sensing portion, the light adjusting portion receiving the light produced by the light source module and providing light of a substantially constant propagation angle to the assay sensing portion, and the assay sensing portion comprising a waveguide.

14. The assay apparatus of claim 13 wherein the light produced by the light source module has a range of propagation angles from angles greater than the minimum angle necessary to intercept the ray redirection portion to angles less than or equal to the maximum angle produced by the light source module.

15. The assay apparatus of claim 13 wherein the assay sensing portion of the sensor includes a waveguide having a coating of sensor molecules, the light of the substantially constant propagation angle passing through the waveguide and creating an evanescent electric field in the coating of sensor molecules.

16. The assay apparatus of claim 13 wherein the assay sensing portion of the sensor includes an optical fiber core having a coating of sensor molecules, the light of the substantially constant propagation angle passing through the optical fiber core and creating an evanescent electric field in the coating of sensor molecules.

17. The assay apparatus of claim 13 wherein the assay sensing portion of the sensor includes a waveguide having a thin metallic coating, the light of the substantially constant propagation angle passing through the waveguide and exciting a surface plasmon wave on a surface of the metallic coating.

18. The assay apparatus of claim 13 wherein the assay sensing portion of the sensor includes an optical fiber core having a thin metallic coating, the light of the substantially constant propagation angle passing through the optical fiber core and exciting a surface plasmon wave on a surface of the metallic coating.

19. The assay system of claim 13, further comprising an interrogation module coupling the light source module with the sensor, the interrogation module including a window containing a waveguide, the waveguide transmitting the light produced by the light source module to the sensor.

20. The assay system of claim 13 wherein the light produced by the light source module is visible light.

21. An assay sensor, comprising:

a propagation angle modifier operable to receive the light having the range of propagation angles and to produce light having a substantially constant propagation angle; and

a waveguide coupled with the propagation angle modifier and operable to receive the light having the substantially constant propagation angle.

22. The sensor of claim 21 wherein the propagation angle modifier and the waveguide are of one-piece construction.

23. The sensor of claim 21 wherein the propagation angle modifier includes a reflecting surface operable to receive as incident light the light having the range of propagation angles and to produce as reflected light the light having the substantially constant propagation angle.

24. The sensor of claim 23 wherein the propagation angle modifier further includes a refracting portion for refracting the light having the range of propagation angles prior to incidence upon the reflecting surface.

25. The sensor of claim 23 wherein the reflecting surface corresponds to a curve described in a polar coordinate system as ##EQU2##

26. The sensor of claim 21 wherein the substantially constant propagation angle corresponds to a critical angle of the waveguide.

27. The sensor of claim 21 wherein the substantially constant propagation angle is approximately less than a critical propagation angle of the waveguide.

28. The sensor of claim 21 wherein the light having a substantially constant propagation angle is light having a narrow range of propagation angles with an average propagation angle corresponding to the substantially constant propagation angle.

29. The sensor of claim 21 wherein the waveguide is a portion of an optical fiber.

30. The sensor of claim 21 wherein the light having a substantially constant propagation angle includes light having a wavelength in an optical portion of an electromagnetic wave spectrum.

31. An optical assay apparatus for detecting analytes in a sample, the apparatus comprising:

a light source operable to produce excitation light; and

a sensor positioned in proximity to the sample, the sensor being coupled with the light source to receive the excitation light and to correspondingly produce an electric field within a portion of the sample to cause production of the emitted light; and

an interrogation module coupling the sensor with the light source and including a window through which the emitted light passes into the interrogation module, the window having a groove in which is positioned a waveguide carrying the excitation light.

32. The optical assay apparatus of claim 31 wherein the groove includes a light absorbing material in which the waveguide is embedded.

33. The optical assay apparatus of claim 31 wherein the waveguide includes an end portion from which the excitation light incident upon the sensor emerges.

34. The optical assay apparatus of claim 33 wherein the end portion includes a reflective surface.

35. The optical assay apparatus of claim 33 wherein the end portion includes a right-angle reflector.

36. The optical assay apparatus of claim 33 wherein the window further includes an obscuration positioned with respect to the end portion to substantially block back-reflected excitation light from passing into the interrogation module.

37. An optical device having an optical axis comprising a reflective surface operable to receive, as incident light, light directed at a plurality of propagation angles relative to the optical axis and to produce, as reflected light, light directed at a substantially constant propagation angle, wherein the reflective surface has a shape corresponding to a curve described in a polar coordinate system as ##EQU3##

38. An optical device having an optical axis, comprising a reflective surface operable to receive, as incident light, light directed at a plurality of propagation angles relative to the optical axis and to produce, as reflected light, light directed at a substantially constant propagation angle, wherein the reflective surface comprises a two-dimensional shape disposed symmetrically about the optical axis and described in the polar coordinate system as

39. An optical device having an optical axis, comprising a reflective surface operable to receive, as incident light, light directed at a plurality of propagation angles relative to the optical axis and to produce, as reflected light, light directed at a substantially constant propagation angle, wherein the reflective surface comprises a three-dimensional shape disposed symmetrically about the optical axis and described in the polar coordinate system as