Apparatus for producing electrophoresis gels

• US 6,139,709 A
• Filed: 03/02/1998
• Issued: 10/31/2000
• Est. Priority Date: 03/02/1998
• Status: Expired due to Fees

• Current Assignee: C.B.S. Scientific Co., Inc. (2invest AG)
• Original Assignee: C.B.S. Scientific Co., Inc. (2invest AG)
• Inventors: Scott, Charles B.
• Primary Examiner(s): Tung, T.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/033,388
• Time in Patent Office: 974 Days
• Field of Search: 204/606, 204/615, 204/616, 204/618, 204/619, 204/620, 71/64.04, 435/397, 99/432, 99/426, 99/430, 99/431, 249/117, 249/134
• US Class Current: 204/619
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44743 Introducing samples

Abstract

A device and apparatus for producing ultra-thin electrophoresis gels formed between spaced-apart plates is provided, where at least one of the plates has a plurality of grooves, the device comprising a spacer comb having extensions formed thereon which are arranged, sized, shaped and located so as to approximately mate with and occupy the grooves on the plate so as to exclude gel material from the grooves during the formation of the electrophoresis gel.

Claims

1. A device for forming sample well access channels in an electrophoresis gel which is formed between spaced-apart plates, at least one of the plates having formed in it a plurality of grooves, said device comprising a spacer comb corresponding in thickness to the approximate thickness of the electrophoresis gel, the spacer comb having extensions formed thereon which are arranged, sized, shaped and located so as to approximately mate with and occupy the grooves on the plate so as to exclude gel material from the grooves during the formation of the electrophoresis gel.

2. The device of claim 1 wherein said grooves are conical in cross section.

3. An apparatus for forming electrophoresis gels comprising

a back plate and a front plate, at least one of the plates having formed in it a plurality of extended grooves, and said plates arranged so that the grooves face toward the remaining plate;

at least one spacer located between the front plate and the back plate and in contact with each of them so that the spacer defines the distance between the plates, the spacer and the plates defining a gel mold there-between; and

a spacer comb located between the front plate and the back plate and corresponding in thickness to the approximate thickness of the spacer, the spacer comb having extensions formed thereon which are arranged, sized, shaped and located so as to approximately mate with and occupy the grooves on the plate so as to exclude gel material from the grooves during the formation of the electrophoresis gel.