×

Squarate dyes and their use in fluorescent sequencing method

  • US 6,140,494 A
  • Filed: 05/12/1999
  • Issued: 10/31/2000
  • Est. Priority Date: 04/19/1996
  • Status: Expired due to Fees
First Claim
Patent Images

1. A squarate dye of the formula (I) or (II) or (IIa) ##STR15## where each Z is independently O or S or CR12,n=1-3,each of s and m is 0, 1 or 2,R1 is lower alkyl (1-4 carbon chain),each R2 is independently selected from electron donating and electron withdrawing groups or is a branched or straight chain of up to 30 carbon atoms incorporating one to five positively charged nitrogen atoms,each R3 is independently selected from:

  • alkylene, alkenylene and alkynylene (1-20 carbon chain), or is a branched or straight chain of up to 30 carbon atoms incorporating one to five ether oxygen atoms or arylene rings or positively charged nitrogen atoms,at least one X is a nucleophilic functional group, such as OH, SH or NH2, or alternatively a grouping capable of reacting with a nucleophile,and any other X present is independently selected from H and SO3- and the residue of a squarate dye of formula (I) or (II) or (IIa) and another fluorochrome,provided that at least one R2 is SO3- and/or at least one X is phosphoramidite.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×