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Method and apparatus for detection of fluorescently labeled materials

  • US 6,141,096 A
  • Filed: 03/25/1997
  • Issued: 10/31/2000
  • Est. Priority Date: 02/10/1994
  • Status: Expired due to Term
First Claim
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1. An apparatus for detecting fluorescently marked regions on a surface of a substrate, said apparatus comprising:

  • a light source;

    an optical train for directing light from said light source at said substrate;

    a focusing system for focusing said light onto a first surface of said substrate;

    a detector for detecting fluoresced light from said fluorescently marked regions in response to said light; and

    a flow cell comprising a mounting surface with a cavity therein having said substrate thereon, wherein there is a sealed relationship between said mounting surface and said substrate, whereby said surface of said substrate is in fluid communication with said cavity, said cavity having an inlet and an outlet, and said inlet being connected to a pump for transferring materials into said cavity through said inlet and out of said cavity through said outlet.

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