One-step method for attachment of biomolecules to substrate surfaces
First Claim
1. A method of making a medical device having a biomolecule immobilized on a substrate surface of a solid polymeric material containing less than 10% water, the method comprising contacting the substrate surface with a reaction mixture comprising a biomolecule, a source of oxidizing metal ions, and an ethylenically unsaturated monomer, the contacting step occurring tinder conditions effective to immobilize the biomolecule on the substrate surface in a one-step reaction.
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Abstract
A method of modifying the surface characteristics of a substrate, particularly a polymeric material. The method involves grafting ethylenically unsaturated monomers and attaching biomolecules, such as heparin, to the surface of the substrate, such as a polymeric material, in one step using an oxidizing metal, such as ceric ions.
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Citations
30 Claims
- 1. A method of making a medical device having a biomolecule immobilized on a substrate surface of a solid polymeric material containing less than 10% water, the method comprising contacting the substrate surface with a reaction mixture comprising a biomolecule, a source of oxidizing metal ions, and an ethylenically unsaturated monomer, the contacting step occurring tinder conditions effective to immobilize the biomolecule on the substrate surface in a one-step reaction.
- 22. A method of modifying the surface characteristics of a solid polymeric material containing less than 10% water, the solid polymeric material comprising a substrate surface, the method comprising contacting the substrate surface of the solid polymeric material with a reaction mixture comprising a biomolecule, oxidizing metal ions, and an ethylenically unsaturated monomer, the contacting step occurring under conditions effective to immobilize the biomolecule on the substrate surface in a one-step process.
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27. A method of modifying the surface characteristics of a metal surface coated with a vinylsilane, the method comprising contacting the metal surface coated with a vinylsilane with a reaction mixture comprising a biomolecule, oxidizing metal ions, and an ethylenically unsaturated monomer, the contacting step occurring under conditions effective to immobilize the biomolecule on the surface in a one-step process.
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28. A method of delivering a biologically active agent, the method comprising contacting a substrate surface of a solid polymeric material containing less than 10% water with a reaction mixture comprising the biologically active agent, oxidizing metal ions, and an ethylenically unsaturated monomer under conditions effective to immobilize the biologically active agent on the substrate surface in a one-step reaction process;
- and contacting the substrate surface having the biologically active agent attached thereto to a physiological solution under conditions effective to release the biologically active agent.
Specification