Cyanidin compositions and therapeutic and diagnostic uses therefor

• US 6,146,837 A
• Filed: 11/23/1998
• Issued: 11/14/2000
• Est. Priority Date: 09/06/1996
• Status: Expired due to Fees

• Current Assignee: Medarex Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Medarex Incorporated (Bristol-Myers Squibb Company)
• Inventors: van de Winkel, Jan G. J.
• Primary Examiner(s): Chan, Christina Y.
• Assistant Examiner(s): LUBET, MARTHA T
• Application Number:
  US09/197,683
• Time in Patent Office: 722 Days
• Field of Search: 435/7.1, 548/427, 548/455, 530/370, 530/387.1, 530/388.22, 530/388.9, 530/388.7
• US Class Current: 435/7.1
• CPC Class Codes: A61K 31/35 having six-membered rings with one oxygen as the only ring hetero atom
• CPC Class Codes: A61K 31/40 having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
• CPC Class Codes: A61K 38/177 Receptors; Cell surface antigens; Cell surface determinants
• CPC Class Codes: A61K 39/385 Haptens or antigens, bound to carriers
• CPC Class Codes: A61K 45/06 Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 31/18 for HIV
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/06 Immunosuppressants, e.g. drugs for graft rejection
• CPC Class Codes: A61P 37/08 Antiallergic agents antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14
• CPC Class Codes: A61P 7/00 Drugs for disorders of the blood or the extracellular fluid
• CPC Class Codes: C07K 14/70535 Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
• CPC Class Codes: G01N 2333/70535 Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
• CPC Class Codes: G01N 33/56972 White blood cells
• CPC Class Codes: G01N 33/6854 Immunoglobulins

Abstract

Compositions comprising cyanidin reagents for binding to FcγRI receptors, and methods and kits for use therefor are provided.

Claims

1. A method for detecting Fcγ

RI (CD64) in a biological sample from a subject, comprising the steps of;

a) contacting the sample with a cyanin compound, Cy5, in the absence of other compounds that bind Fcγ

RI;

b) illuminating the sample with light of an appropriate excitation wavelength for Cy5; and

c) detecting fluorescence.

2. The method of claim 1, wherein the Cy5 further comprises an additional dye reagent.

3. The method of claim 2, wherein the additional dye reagent is a fluorescein reagent or a phycoerythrin reagent.

4. The method of claim 1, wherein the step of detecting fluorescence is done by flow cytometry.

5. The method of claim 1, wherein the step of detecting fluorescence is done by fluorescent microscopy.

6. The method of claim 1, wherein the excitation wavelength is approximately 475 to 505 nm.

7. The method of claim 1, wherein the sample is obtained from a human subject.

8. The method of claim 1, wherein the level of fluorescence of the sample, is compared to the level of fluorescence of a second biological sample from a different subject.

9. The method of claim 1, wherein a therapeutic agent is administered to the subject prior to the step of detecting.

10. The method of claim 9, wherein the therapeutic agent is selected from the group consisting of an interferon, an interleukin, and a colony stimulating factor.

11. The method of claim 10, wherein the interferon is IFN-γ

.

12. The method of claim 10, wherein the interleukin is IL-10.

13. The method of claim 10, wherein the colony stimulating factor is G-CSF.

14. The method of claim 1, further comprising the step of removing unbound Cy5 before illuminating the sample with light of an appropriate excitation wavelength.