×

Filtration processes, kits and devices for isolating plasmids

  • US 6,146,854 A
  • Filed: 08/31/1995
  • Issued: 11/14/2000
  • Est. Priority Date: 08/31/1995
  • Status: Expired due to Term
First Claim
Patent Images

1. A process for isolating plasmid DNA from plasmid-containing cells, comprising the steps of:

  • a) washing plasmid-containing cells by filtration with a wash solution to disrupt the cell membrane and degrade RNA;

    b) incubating the resulting washed plasmid-containing cells with a lysis/denaturation solution to lyse the cells and denature DNA;

    c) incubating the product of step b) with a renaturation solution for an appropriate period of time and at an appropriate temperature to yield a renatured mixture containing dissolved plasmid DNA, insoluble linear DNA and cellular debris;

    d) filtering the product of step c) to obtain a filtrate from which insoluble linear DNA and cellular debris have been removed; and

    e) obtaining plasmid DNA from the filtrate, wherein no centrifugation step is used in the process.

View all claims
  • 4 Assignments
Timeline View
Assignment View
    ×
    ×