Microfluidic devices incorporating improved channel geometries

• US 6,153,073 A
• Filed: 08/11/1999
• Issued: 11/28/2000
• Est. Priority Date: 04/25/1997
• Status: Expired due to Term

• Current Assignee: Caliper Life Sciences Incorporated (Revvity, Inc.)
• Original Assignee: Caliper Technologies Corp (Revvity, Inc.)
• Inventors: Kennedy, Colin B., Dubrow, Robert S., Bousse, Luc J.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): STARSIAK, JOHN S
• Application Number:
  US09/372,655
• Time in Patent Office: 475 Days
• Field of Search: 204/450, 204/451, 204/452, 204/453, 204/454, 204/455, 204/600, 204/601, 204/602, 204/603, 204/604, 204/605, 422/100, 422/99, 422/68.1, 422/70, 422/102, 435/287.1, 435/287.2, 435/287.3, 435/288.5, 435/288.6, 435/288.7
• US Class Current: 204/453
• CPC Class Codes: B01L 2200/0605 Metering of fluids
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2400/0418 electro-osmotic flow [EOF]
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027
• CPC Class Codes: Y10S 366/02 Micromixers: segmented laminar flow with boundary mixing orthogonal to the direction of fluid propagation with or without geometry influences from the pathway
• CPC Class Codes: Y10S 366/03 Micromixers: variable geometry from the pathway influences mixing/agitation of non-laminar fluid flow

Abstract

The present invention generally provides microfluidic devices which incorporate improved channel and reservoir geometries, as well as methods of using these devices in the analysis, preparation, or other manipulation of fluid borne materials, to achieve higher throughputs of such materials through these devices, with lower cost, material and/or space requirements.

Claims

1. A microfluidic device, comprising:

a body structure comprising a main channel and a sample loading channel fabricated therein, the main channel intersecting the sample loading channel, which sample loading channel is fluidly coupled to a source of at least one sample material and a fluid reservoir; and

,

a transport system coupled to the sample loading channel, the transport system comprising one or more control devices which direct movement of the at least one sample material through the sample loading channel to a position proximal to the intersection of the sample loading channel and the main channel, which one or more control devices concomitantly direct flow of material through the main channel.

2. The microfluidic device of claim 1, further comprising, an instruction set for injecting the at least one sample material into the main channel and electroosmotically or electrophoretically flowing the resulting injected sample through at least a portion of the main channel while simultaneously pre-loading an additional sample into the sample loading channel.

3. The microfluidic device of claim 1, further comprising an instruction set for injecting the additional sample into the main channel and electroosmotically or electrophoretically flowing the resulting injected additional sample through at least a portion of the main channel while simultaneously pre-loading a further additional sample into the sample loading channel.

4. The microfluidic device of claim 1, wherein the loading channel is fluidly connected to a plurality of reservoirs comprising at least three reservoirs, which at least three reservoirs are arranged on a surface of the body structure at regularly spaced intervals, wherein two of the at least three reservoirs are disposed on a first side of the main channel and one of the at lest three reservoirs are disposed on a second side of the main channel and wherein at least one of the at least three reservoirs corresponds to the source of at least one sample material or the fluid reservoir.

5. A microfluidic device of claim 4, comprising at least six reservoirs arranged on the surface of the body structure at regularly spaced intervals, which reservoirs are fluidly connected to the loading channel and wherein at least one of the at least six reservoirs corresponds to the source of at least one sample material or the fluid reservoir.

6. The microfluidic device of claim 5, wherein three of the at least six reservoirs are disposed on a first side of the main channel and three of the at least six reservoirs are disposed on a second side of the main channel.

7. The microfluidic device of claim 4, comprising at least eight reservoirs arranged on the surface of the body structure at regularly spaced intervals, which reservoirs are fluidly connected to the loading channel and wherein at least one of the at least eight reservoirs corresponds to the source of at least one sample material or the fluid reservoir.

8. The microfluidic device of claim 7, wherein four of the at least eight reservoirs are disposed on a first side of the main channel and four of the at least eight reservoirs are disposed on a second side of the main channel.

9. The microfluidic device of claim 4, wherein the reservoirs of the device are arranged on approximately 9 mm centers.

10. The microfluidic device of claim 4, wherein the reservoirs of the device are arranged on approximately 4.5 mm centers.

11. The microfluidic device of claim 4, wherein the reservoirs of the device are arranged on approximately 2.25 mm centers.

12. The microfluidic device of claim 4, wherein the reservoirs of the device are arranged at a density greater than about 2 reservoirs per centimeter².

13. The microfluidic device of claim 4, wherein the reservoirs of the device are arranged at a density greater than about 4 reservoirs per centimeter².

14. The microfluidic device of claim 4, wherein the reservoirs of the device are arranged at a density greater than about 8 reservoirs per centimeter².

15. The microfluidic device of claim 4, comprising at least four reservoirs substantially equidistant from the intersection of the loading channel and the main channel.

16. The microfluidic device of claim 15 further comprising at least one electrode in contact with each of the at least four wells.

17. The microfluidic device of claim 4, wherein the channels of the device are approximately the same width and depth.

18. The microfluidic device of claim 4, wherein the channels of the device comprise at least one dimension between about 1 and 500 microns.

19. The microfluidic device of claim 4, wherein the channels of the device have a depth of about 12 microns and a width of between about 30 and 70 microns.

20. The microfluidic device of claim 1, further comprising at least one pre-loading module which comprises a sample reservoir fluidly connected to the loading channel and a waste reservoir fluidly connected to the loading channel, wherein, during operation of the device, material is flowed from the sample reservoir to a position in the loading channel, which position is proximal to the intersection of the loading channel and the main channel, by applying a voltage difference between the waste well and the sample well, wherein movement of the material to the position proximal to the intersection is made substantially without affecting movement of a second material in the main channel.

21. The microfluidic device of claim 1, wherein the body structure is fabricated form one or more of:

a silica based substrate and a polymeric material.

22. The microfluidic device of claim 1, wherein the body structure is fabricated from one or more of:

a polydimethylsiloxane, a polymethylmethacrylate, a polyurethane, a polyvinylchloride, a polystyrene polysulfone, a polycarbonate, a polymethylpentene, a polypropylene, a polyethylene, a polyvinylidine fluoride, ABS, glass, quartz, silicon and polysilicon.

23. The microfluidic device of claim 1, wherein the body structure is fabricated from a first substrate having grooves fabricated thereon, which first substrate is overlaid by a second substrate, wherein the first and second substrates are fabricated from a material independently selected from:

a polydimethylsiloxane, a polymethylmethacrylate, a polyurethane, a polyvinylchloride, a polystyrene polysulfone, a polycarbonate, a polymethylpentene, a polypropylene, a polyethylene, a polyvinylidine fluoride, ABS, glass, quartz, silicon and polysilicon.

24. The microfluidic device of claim 23, wherein the grooves are embossed or etched on the first substrate.

25. The microfluidic device of claim 1, wherein the main channel comprises a separation medium disposed therein.

26. The microfluidic device of claim 1, wherein the main channel comprises a sieving matrix disposed therein.

27. The microfluidic device of claim 1, wherein the one or more control devices include one or more of:

a voltage regulator which regulates voltage between the sample material source and the fluid reservoir;

a pressure regulator which regulates pressure at the sample material source or the fluid reservoir;

or,

a hydrodynamic force regulator which regulates hydrodynamic force at the sample material source or the fluid reservoir.