NES-1 polypeptides, DNA, and related molecules and methods

• US 6,153,387 A
• Filed: 11/30/1998
• Issued: 11/28/2000
• Est. Priority Date: 06/06/1995
• Status: Expired due to Fees

• Current Assignee: New England Medical Center Hospitals, Inc.
• Original Assignee: New England Medical Center Hospitals, Inc.
• Inventors: Band, Vimla
• Primary Examiner(s): Nashed, Nashaat T.
• Application Number:
  US09/201,038
• Time in Patent Office: 729 Days
• Field of Search: 435/212, 435/219, 435/320, 435/252.33, 435/213, 435/226, 435/6, 435/325, 435/254.11, 435/320.1, 435/69.1, 530/350, 530/300, 536/23.2, 536/23.5
• US Class Current: 435/6.14
• CPC Class Codes: C12N 9/6424 Serine endopeptidases (3.4.21)

Abstract

Disclosed is substantially pure NES1 polypeptide and purified DNA, vectors, and cells encoding that polypeptide. Also disclosed are methods for carcinoma detection and treatment using the NES1 sequence.

Claims

1. An isolated nucleic molecule comprising a nucleotide sequence encoding the amino acid sequence of SEQ ID NO. 1.

5. A vector comprising the nucleic acid molecule of any of claims 1 and 2-4.

6. A host cell comprising the vector of claim 5.

7. A host cell comprising the isolated nucleic acid molecule of any of claims 1 and 2-4.

8. A method of making the polypeptide of SEQ ID NO:

1 comprising culturing the host cell of claim 6, and isolating the said polypeptide.

9. A method of making the polypeptide of SEQ ID NO:

1 comprising culturing the host cell of claim 7, and isolating the said polypeptide.

2. An isolated nucleic acid molecule consisting of a fragment of the nucleic acid molecule of SEQ ID NO:

2 wherein said fragment comprises at least 50 contiguous nucleotides of SEQ ID NO;

2.

3. The nucleic acid molecule of claim 2, wherein the fragment comprises at least 110 contiguous nucleotides of SEQ ID NO:

2.

4. An isolated nucleic acid molecule, said molecule comprising a nucleotide sequence encoding a polypeptide consisting of an amino acid sequence selected from the group consisting of amino acids 1-48, 120-136, 86-245, 42-245, 180-276, 121-137, and 1-20 of SEQ ID NO:

1.

10. A method for detecting mRNA encoding the amino acid sequence of SEQ ID NO:

1 in a biological sample, the method comprising;

a. providing a test biological sample,

b. contacting the test biological sample with an isolated nucleic acid molecule that hybridizes under high stringency conditions to a nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO;

2, and

c. determining that mRNA encoding the amino acid sequence of SEQ ID NO;

1 is present in the sample when the test biological sample contains mRNA that hybridizes the isolated nucleic acid molecule.

12. The method of claim 10 or 11 wherein the test sample comprises epithelial cells.

13. The method of claim 12 wherein the test sample comprises breast tissue.

14. The method of claim 12 wherein the sample comprises cervical tissue.

11. A method of detecting a malignancy or an increased likelihood of developing a malignancy in a patient, the method comprising:

a. obtaining a test sample from a tissue of the patient;

b. obtaining a control sample of known normal cells from the same type of tissue as the test sample;

c. contacting the biological sample with an isolated nucleic acid molecule that hybridizes under high stringency conditions to a nucleic acid molecule consisting of the nucleotide sequence of SEQ ID NO;

2

d. measuring the relative amount of mRNA encoding the amino acid sequence of SEQ ID NO;

1 in the test sample and the control sample; and

e. determining that the patient has a malignancy or has an increased likelihood of developing a malignancy if the amount of mRNA encoding the amino acid sequence of SEQ ID NO;

1 in the test sample is less than the amount of mRNA in the control sample.