Waveguide detection of right-angle-scattered light in flow cytometry
First Claim
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1. A flow cytometer flow cell, comprising:
- a transparent flow cell having a hollow flow capillary to allow the flow of a sheath liquid therethrough, wherein said transparent flow cell comprises a solid-state material having an index of refraction that is greater than that of said sheath liquid, wherein particles suspended in said sheath liquid will produce scattered light from a laser beam directed at said particles, wherein a first portion of said scattered light will propagate into said solid-state material of said transparent flow cell; and
a detector positioned to detect a second portion of said first portion of said scattered light.
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Abstract
A transparent flow cell is used as an index-guided optical waveguide. A detector for the flow cell but not the liquid stream detects the Right-Angle-Scattered (RAS) Light exiting from one end of the flow cell. The detector(s) could view the trapped RAS light from the flow cell either directly or through intermediate optical light guides. If the light exits one end of the flow cell, then the other end of the flow cell can be given a high-reflectivity coating to approximately double the amount of light collected. This system is more robust in its alignment than the traditional flow cytometry systems which use imaging optics, such as microscope objectives.
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Citations
37 Claims
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1. A flow cytometer flow cell, comprising:
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a transparent flow cell having a hollow flow capillary to allow the flow of a sheath liquid therethrough, wherein said transparent flow cell comprises a solid-state material having an index of refraction that is greater than that of said sheath liquid, wherein particles suspended in said sheath liquid will produce scattered light from a laser beam directed at said particles, wherein a first portion of said scattered light will propagate into said solid-state material of said transparent flow cell; and a detector positioned to detect a second portion of said first portion of said scattered light. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37)
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Specification