Enzyme electrode structure

• US 6,156,173 A
• Filed: 01/18/2000
• Issued: 12/05/2000
• Est. Priority Date: 09/12/1997
• Status: Expired due to Term

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: NOK Corporation
• Inventors: Mure, Hiroki, Shirakawa, Hiroshi, Gotoh, Masao
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US09/484,539
• Time in Patent Office: 322 Days
• Field of Search: 435/287.1, 435/287.2, 435/287.9, 435/288.3, 435/817, 204/403, 422/68.1, 422/82.01, 422/82.02, 422/82.05, 422/82.09, 422/102, 422/100, 356/246
• US Class Current: 204/403.04
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A biosensor comprises a space part for sucking and housing a sample formed of two upper and lower plates, the two plates being stuck together by an adhesive layer, the space part for sucking and housing the sample being constituted so as to be partially opened in the peripheral part and partially closed by the adhesive layer, and has a working electrode having at least glucose oxidase immobilized thereon and a counter electrode on the same plane of the plate.

Claims

1. A biosensor comprising an upper plate, a lower plate, a space formed between the two plates for sucking and housing a sample therein, a layer for fixing the two plates together, the space for sucking and housing the sample being partially opened at a peripheral part thereof and partially closed by the layer, a working electrode and a counter electrode fixed relative to the working electrode, each of the two plates lacking an air vent or hole for connecting to the space, one end of each of the two plates having a tapered part, a tip part of the working electrode or a tip part of the counter electrode provided on the tapered part.

2. A biosensor according to claim 1, wherein the working electrode has at least an oxidoreductase.

3. A biosensor according to claim 1, wherein the working electrode and the counter electrode are formed on the upper and lower plates, respectively, so as to face each other.

4. A biosensor according to claim 1, wherein an oxidoreductase-electron transmitter mixture layer is provided on the working electrode.

5. A biosensor according to claim 1, wherein the working electrode includes glucose oxidase.

6. A biosensor according to claim 5, wherein the glucose oxidase is fixed to the working electrode by drying.

7. A biosensor according to claim 1, wherein the layer is an adhesive layer.

8. A biosensor according to claim 7, wherein a spacer with adhesive on both sides thereof is interposed between the two plates.

9. A biosensor according to claim 7, wherein a reference electrode is arranged at a side of the working electrode or the counter electrode.

10. A biosensor according to claim 7, wherein the adhesive layer has two surfaces and an adhesive on at least one of the two surfaces.

11. A biosensor according to claim 7, wherein the adhesive layer has two surfaces and an adhesive on both of the two surfaces.

12. A biosensor according to claim 7, wherein one end of each of the plates is formed into a protruding shape.

13. A biosensor according to claim 7, wherein an oxidoreductase is fixed on the counter electrode.

14. A biosensor according to claim 1, wherein an insulating layer is formed between one of the two plates and the layer.