Method of purification of physiological liquids of organism

• US 6,159,377 A
• Filed: 11/02/1998
• Issued: 12/12/2000
• Est. Priority Date: 02/06/1998
• Status: Expired due to Term

• Current Assignee: Cytosorbents Corporation, Donald Brodie, RenalTech International LLC, Stefan Brodie
• Original Assignee: RenalTech International LLC
• Inventors: Tur, Dzirda, Davankov, Vadim, Pavlova, Ludmila, Tsyurupa, Maria
• Primary Examiner(s): COPENHEAVER, BLAINE R
• Application Number:
  US09/184,166
• Time in Patent Office: 771 Days
• Field of Search: 502/402, 521/30, 521/31, 521/32, 521/55, 521/75, 521/905, 521/918, 521/146, 428/305.5, 428/409, 428/543, 210/767, 210/690
• US Class Current: 210/690
• CPC Class Codes: A61M 1/3627 Degassing devices; Buffer reservoirs; Drip chambers; Blood filters
• CPC Class Codes: B01J 20/26 Synthetic macromolecular compounds
• CPC Class Codes: B01J 20/261 obtained by reactions only involving carbon to carbon unsaturated bonds macromolecular compounds obtained by reactions only involving carbon-to-carbon unsaturated bonds per se C08F
• CPC Class Codes: B01J 20/321 consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3219 involving a particular spacer or linking group, e.g. for attaching an active group
• CPC Class Codes: B01J 20/3272 Polymers obtained by reactions otherwise than involving only carbon to carbon unsaturated bonds
• CPC Class Codes: B01J 20/3274 Proteins, nucleic acids, polysaccharides, antibodies or antigens
• CPC Class Codes: B01J 20/3285 Coating or impregnation layers comprising different type of functional groups or interactions, e.g. different ligands in various parts of the sorbent, mixed mode, dual zone, bimodal, multimodal, ionic or hydrophobic, cationic or anionic, hydrophilic or hydrophobic
• CPC Class Codes: C08F 8/24 Haloalkylation
• CPC Class Codes: Y10S 521/905 Hydrophilic or hydrophobic cellular product
• CPC Class Codes: Y10S 521/918 Physical aftertreatment of a cellular product
• CPC Class Codes: Y10T 428/249954 With chemically effective material or specified gas other than air, N, or carbon dioxide in void-containing component
• CPC Class Codes: Y10T 428/31 Surface property or characteristic of web, sheet or block
• CPC Class Codes: Y10T 428/8305 Miscellaneous [e.g., treated surfaces, etc.]

Abstract

A method of purification of physiological liquids of organism has the step of passing a physiological liquid through a material which has a size, a shape, and a structure selected so as to remove toxic compounds from the physiological liquid and is composed of a partially chloromethylated porous highly crosslinked styrene or divinylbenzene copolymer which initially have surface exposed chloromethyl groups in which thereafter chlorine is replaced with an element which forms different surface exposed functional groups with a greater hydrophilicity and greater biocompatibility than that of the chloromethyl group.

Claims

1. A method of purification of physiological liquids of organism, comprising the step of passing a physiological liquid through a material which has a size, a shape, and a structure selected so as to remove toxic compounds from the physiological liquid and is composed of a partially chloromethylated porous highly crosslinked styrene or divinylbenzene copolymer which initially have surface exposed chloromethyl groups in which thereafter chlorine is replaced with an element which forms different surface exposed functional groups with a greater hydrophilicity and greater biocompatibility than that of the chloromethyl group, wherein the replacement step is limited exclusively to surface exposed groups.

2. A method as defined in claim 1, wherein said polymeric material has polymer beads with mainly unsubstituted hydrophobic interior which is responsible for removing of toxic compounds in a molecular range of 300 to 2000 Dalton.

3. A method as defined in claim 1, wherein said porous highly crosslinked styrene or divinylbenzene copolymer is a macroporous or mesoporous styrene-divinylbenzene-ethylstyrene copolymer subjected to a partial chloromethylation to a chlorine content of up to 7%.

4. A method as defined in claim 1, wherein said porous highly crosslinked styrene or divinylbenzene copolymer is a hypercrosslinked polystyrene produced from crosslinked styrene copolymers by an extensive chloromethylation and a subsequent post-crosslinking by treating with a Friedel-Crafts catalyst in a swollen state.

5. A method as defined in claim 1, wherein said porous highly crosslinked styrene or divinylbenzene copolymer is a hypercrosslinked polystyrene produced from crosslinked styrene copolymers by an extensive additional post-crosslinking in a swollen state with bifunctional crosslinking agents selected from the group consisting of monochlorodimethyl ether and p-xylilene dichloride.

6. A method as defined in claim 1, wherein said surface exposed functional groups of greater hydrophilicity and enhanced biocompatibility are covalently bonded or electrostatically deposited long polymeric chains selected from the group consisting of heparine, polyethyleneglycol, chitozane, and poly-bis(trifluoroethoxy)phosphazene.

7. A method as defined in claim 1, wherein said surface exposed functional groups of greater hydrophilicity and enhanced biocompatibility are covalently bonded lipid-like structures selected from a group consisting of phosphatidyl choline, phosphatidyl serine, and phosphatidyl 2-ethanol amine.