Coating having biological activity and medical implant having surface carrying the same and method

• US 6,159,531 A
• Filed: 08/30/1999
• Issued: 12/12/2000
• Est. Priority Date: 08/30/1999
• Status: Expired due to Fees

• Current Assignee: Covidien LP (Medtronic Plc)
• Original Assignee: CardioVasc, Inc. (Medtronic Plc)
• Inventors: Dang, Mai Huong, Chiu, Phillip
• Primary Examiner(s): Beck, Shrive
• Assistant Examiner(s): MICHENER, JENNIFER KOLB
• Application Number:
  US09/385,692
• Time in Patent Office: 470 Days
• Field of Search: 427/2.24, 427/2.25, 427/2.28, 427/2.3, 427/491, 427/534, 427/535, 427/536, 427/569
• US Class Current: 427/2.24
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 33/0088 by grafting of a monomer onto the substrate
• CPC Class Codes: C08J 7/12 Chemical modification

Abstract

A multi-step method for surface modification of a medical device having at least one surface exposed to tissue and/or blood. The steps comprise a low temperature plasma treatment of the said surface to provide a plasma deposited layer having functional groups. A chemical treatment of the plasma deposited layer using multifunctional linkers establishes covalent bonds with the plasma deposited layer. A bioactive/biocompatible agent react with the multifunctional linkers so that molecules thereof are covalently bound to said surface.

Claims

1. A method for treating a medical device having at least one surface exposed to tissue and/or blood comprising plasma cleaning said one surface with a gaseous plasma process utilizing radio frequency energy to ablate said one surface, wherein the gas used in said plasma cleaning process consists essentially of oxygen, a mixture of oxygen with argon or nitrogen, or air, functionalizing said one surface after said one surface has been cleaned with a plasma deposition to provide a plasma-deposited layer having functional groups and subjecting said plasma-deposited layer to multifunctional liners/spacers in a wet chemical treatment to form covalent bonds between the linkers/spacers and the functional groups of the plasma-deposited layer to provide a durable coating covalently bound to said one surface of the substrate.

2. A method as in claim 1 further including the step of binding a bioactive/biocompatible agent having molecules to the covalently bound linkers spacers so that molecules thereof are covalently bound to said surface.

3. A method as in claim 2 in which the bioactive compound is a cell-adhesion agent.

4. A method as in claim 1 wherein the plasma process is created using a gas having functional groups selected from amine, carboxylic and hydroxyl groups.

5. A method as in claim 1 wherein the plasma process is created using a functional group selected from inorganic and organic compounds.

6. A method as in claim 5 wherein the inorganic compounds are selected from the group consisting of NH₃, N₂ H₄, and H₂ O.

7. A method as in claim 5 wherein the organic compounds include saturated and unsaturated compounds and their derivatives.

8. A method as in claim 7 wherein the saturated compounds are selected from the group:

consisting of aliphatic alcohols, alipathic carboxylic acids and alipathic amines.

9. A method as in claim 7 wherein the unsaturated compounds include allylamine, allyl alcohol, vinyl alcohol, vinyl acetate, acrylic acid and methacrylic acid.

10. A method as in claim 1 further including the step of washing the surface with a rinse selected from an organic solvent rinse and a buffered rinse to wash off loosely bound deposits.

11. A method as in claim 1 wherein the multifunctional linkers are selected from homo and hetero multifunctional linkers.

12. A method as in claim 11 wherein said homo multifunctional linkers are selected from a group comprising dicarboxylic acid anhydrides, dicarboxylic acids, diols and diamines.

13. A method as in claim 11 wherein said hetero multifunctional linkers are selected from amino acids.

14. A method as in claim 11 wherein the multifunctional linkers are comprised of at least one molecule with 2-20 carbon atoms in the backbone.

15. A method as in claim 11 wherein the multifunctional linkers spacers are strings of heterofunctional molecules.

16. A method as in claim 11 wherein said linkers/spacers are strings formed of alternate homofunctional molecules.

17. A method as in claim 12 wherein said homo multifunctional linkers/spacers are succinic anhydride.

18. A method as in claim 1 wherein said plasma cleaning is performed at a higher power than the plasma deposition.

19. A method as in claim 18 wherein the plasma cleaning is carried out at a pressure of 0.1 to 1.0 Torr and with a radiofrequency power of 50-1000 watts and wherein the plasma deposition step is carried out at a lower pressure of 0.05 to 5 Torr and a lower radiofrequency power of 2-300 watts.

20. A method as in claim 1 wherein the multifunctional linkers/spacers are introduced in the presence of a solubility enhancer.

21. A method as in claim 2 wherein the bioactive/biocompatible agent is introduced in the presence of a solubility enhancer.

22. A method as in claim 20 wherein the solubility enhancer is selected from the group consisting of DMSO, DMF and dioxane.