Synthetic HPV6/11 hybrid L1 DNA encoding human papillomavirus type 11 L1 protein

• US 6,159,729 A
• Filed: 01/26/1998
• Issued: 12/12/2000
• Est. Priority Date: 03/30/1995
• Status: Expired due to Term

• Current Assignee: Merck Sharp & Dohme Corporation (Merck & Co., Inc.)
• Original Assignee: Merck & Co., Inc.
• Inventors: Neeper, Michael P., Joyce, Joseph G., George, Hugh A., Lehman, E. Dale, Jansen, Kathrin U., Hofmann, Kathryn J.
• Primary Examiner(s): Degen, Nancy
• Assistant Examiner(s): SCHWARTZMAN, ROBERT A
• Application Number:
  US08/913,462
• Time in Patent Office: 1,051 Days
• Field of Search: 424/186.1, 424/204.1, 435/69.1, 435/69.3, 435/235.1, 435/320.1, 514/2, 514/44, 530/387.9, 530/388.3, 530/389.4, 536/23.1, 536/23.72
• US Class Current: 435/320.1
• CPC Class Codes: A61K 2039/51 comprising whole cells, viruses or DNA/RNA
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 31/20 for DNA viruses
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 2710/20022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Abstract

The present invention is directed to a synthetic DNA molecule encoding purified human papillomavirus type 11 L1 protein and derivatives thereof.

Claims

1. An isolated and purified DNA molecule encoding human papillomavirus type 11 L1 protein, the DNA molecule being free of internal transcription termination signals which are recognized by yeast.

2. The DNA molecule of claim 1 having the sequence shown in FIG. 8 SEQ. ID. NO.38.

3. An expression vector comprising the DNA molecule of claim 1.

4. A composition comprising the DNA molecule of claim 1, or RNA complementary to the DNA molecule of claim 1.

5. An expression vector comprising the DNA molecule of claim 2.

6. The expression vector of claim 5 which is D362-1.

7. A nucleic acid encoding human papillomavirus type 11 L1 protein, the nucleic acid molecule being free from internal transcription termination signals which are recognized by yeast.