Human pinin splice variant

• US 6,162,601 A
• Filed: 08/08/1997
• Issued: 12/19/2000
• Est. Priority Date: 08/08/1997
• Status: Expired due to Fees

• Current Assignee: Incyte Pharmaceuticals, Inc.
• Original Assignee: Incyte Pharmaceuticals, Inc.
• Inventors: Lal, Preeti, Bandman, Olga, Shah, Purvi
• Primary Examiner(s): Guzo, David
• Application Number:
  US08/910,925
• Time in Patent Office: 1,229 Days
• Field of Search: 435/325, 435/366, 435/320.1, 435/69.1, 435/6, 435/91.1, 435/91.2, 536/23.1, 536/23.5, 536/24.3
• US Class Current: 435/6.16
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/158 Expression markers

Abstract

The invention provides a human pinin splice variant (PNIN) and polynucleotides which identify and encode PNIN. The invention also provides expression vectors, host cells, agonists, antibodies and antagonists. The invention also provides methods for treating disorders associated with expression of PNIN.

Claims

1. An isolated and purified polynucleotide sequence encoding the human pinin splice variant of SEQ ID NO:

1.

2. An isolated and purified polynucleotide sequence which is completely complementary to the polynucleotide sequence of claim 1.

3. An expression vector comprising the polynucleotide of claim 1.

4. A method for detecting a polynucleotide which encodes human pinin splice variant in a biological sample comprising the steps of:

a) hybridizing the polynucleotide of claim 2 to nucleic acid material of a biological sample, thereby forming a hybridization complex; and

b) detecting said hybridization complex, wherein the presence of said complex correlates with the presence of a polynucleotide encoding human pinin splice variant in said biological sample.

5. The method of claim 4 wherein the nucleic acid material is amplified by the polymerase chain reaction prior to hybridization.

8. A host cell containing the vector of claim 3.

9. A method for producing a polypeptide comprising the amino acid sequence of SEQ ID NO:

1, the method comprising the steps of;

a) culturing the host cell of claim 8 under conditions suitable for the expression of the polypeptide; and

b) recovering the polypeptide from the host cell culture.

6. An isolated and purified polynucleotide sequence comprising SEQ ID NO:

2.

7. An isolated and purified polynucleotide sequence which is completely complementary to the polynucleotide sequence of claim 6.