Methods of making polymeric arrays
First Claim
1. A method of making an array of at least two distinct polymeric compounds of differing composition covalently bonded to a surface of a solid support, said method comprising:
- contacting a surface of a solid support having a cycloaddition reactive group and a contact angle ranging from about 20°
to 100°
with said distinct polymeric compounds under conditions sufficient for said polymeric compounds to covalently bond to said surface by a cycloaddition reaction.
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Accused Products
Abstract
Methods are provided for making arrays of distinct polymers covalently bonded to the surface of the a solid support. In the subject methods, at least two distinct polymers, e.g. nucleic acids, are contacted with the surface of a solid support under conditions sufficient for the nucleic acids to become covalently bonded to the surface of the solid support through a cycloaddition reaction, e.g. through the reaction of a diene with a dienophile. Also provided are arrays produced by the subject methods, kits comprising the same and methods for using the arrays in analyte detection, e.g. hybridization, assays.
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Citations
32 Claims
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1. A method of making an array of at least two distinct polymeric compounds of differing composition covalently bonded to a surface of a solid support, said method comprising:
contacting a surface of a solid support having a cycloaddition reactive group and a contact angle ranging from about 20°
to 100°
with said distinct polymeric compounds under conditions sufficient for said polymeric compounds to covalently bond to said surface by a cycloaddition reaction.- View Dependent Claims (2, 3, 4, 29)
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5. A method of producing an array of at least two distinct nucleic acids that differ by sequence covalently bonded to the surface of a solid support, said method comprising:
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contacting a surface of a solid support having a cycloaddition reactive group and a contact angle ranging from about 20 to 100°
with said nucleic acids under conditions sufficient for said nucleic acids to covalently bond to said surface by a Diels-Alder reaction;
whereby said array of nucleic acids is produced. - View Dependent Claims (6, 7, 8, 9)
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10. A method of producing an array of at least two distinct polydeoxyribonucleotides that differ by sequence covalently bonded to the surface of a solid support, said method comprising:
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contacting a surface of a solid support having a cycloaddition reactive group and a contact angle ranging from about 20 to 100°
with said polydeoxyribonulcucleotides under conditions sufficient for said nucleic acids to covalently bond to said surface by a Diels-Alder reaction, wherein a terminus of said polydeoxyribonucleotides comprises a diene and said surface comprises a dienophile;
whereby said array of nucleic acids is produced. - View Dependent Claims (11, 12, 13, 30)
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14. A method of making a polymeric array of at least:
- (a) a first spot having a diameter ranging from 10 to 1000 μ
m and made up of a first polymer covalently bound to a surface; and
(b) a second spot having a diameter ranging from 10 to 1000 μ
m and made up of a second polymer covalently bound to said surface of said solid support;
said method comprising;depositing;
(a) from about 1 nl to 1 pl of a first fluid composition comprising said first polymer onto a surface having a cycloaddition reactive group and a contact angle ranging from about 20 to 100°
to produce said first spot having a diameter ranging from 10 to 1000 μ
m; and
(b) from about 1 nl to 1 pl of a second fluid comprising said second polymer onto said surface to produce said second spot having a diameter ranging from 10 to 1000 μ
m,wherein said first and second fluid compositions are deposited onto said surface under conditions sufficient for said first and second polymeric compounds to covalently bond to said surface by a cycloaddition reaction;
whereby said polymeric array is produced. - View Dependent Claims (15, 16, 17, 31)
- (a) a first spot having a diameter ranging from 10 to 1000 μ
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18. A method of making a nucleic acid array of at least:
- (a) a first spot having a diameter ranging from 10 to 1000 μ
m and made up of a first nucleic acid covalently bound to a surface; and
(b) a second spot having a diameter ranging from 10 to 1000 μ
m and made up of a second nucleic acid covalently bound to said surface of said solid support;
said method comprising;depositing;
(a) from about 1 nl to 1 pl of a first fluid composition comprising said first nucleic acid onto a surface having a cycloaddition reactive group and a contact angle ranging from about 20 to 100°
to produce said first spot having a diameter ranging from 10 to 1000 μ
m; and
(b) from about 1 nl to 1 pl of a second fluid comprising said second nucleic acid onto said surface to produce said second spot having a diameter ranging from 10 to 1000 μ
m,wherein said first and second fluid compositions are deposited onto said surface under conditions sufficient for said first and second nucleic acids to covalently bond to said surface by Diels-Alder reaction;
whereby said array of nucleic acids is produced. - View Dependent Claims (19, 20, 21, 22, 23)
- (a) a first spot having a diameter ranging from 10 to 1000 μ
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24. A method of making a polydeoxyribonucleotide array of at least:
- (a) a first spot having a diameter ranging from 10 to 1000 μ
m and made up of a first polydeoxyribonucleotide covalently bound to a surface; and
(b) a second spot having a diameter ranging from 10 to 1000 μ
m and made up of a second polydeoxyribonucleotide covalently bound to said surface of said solid support;
said method comprising;depositing;
(a) from about 1 nl to 1 pl of a first fluid composition comprising said first polydeoxyribonucleotide onto a surface having a cycloaddition reactive group and a contact angle ranging from about 20 to 100°
to produce said first spot having a diameter ranging from 10 to 1000 μ
m; and
(b) from about 1 nl to 1 pl of a second fluid comprising said second polydeoxyribonucleotide onto said surface to produce said second spot having a diameter ranging from 10 to 1000 μ
m,wherein said first and second fluid compositions are deposited onto said surface under conditions sufficient for said first and second polydeoxyribonucleotide to covalently bond to said surface by a Diels-Alder reaction;
whereby said array of polydeoxyribonucleotides is produced. - View Dependent Claims (25, 26, 27, 28, 32)
- (a) a first spot having a diameter ranging from 10 to 1000 μ
Specification