Process and device for determining the activity of enzymes in liquids, or the concentration and/or activity of inhibitors in liquids
First Claim
1. A device for measuring the activity of an enzyme in liquid, the device comprising a flow through column and a detector, the column including a chromatographic carrier having a substance capable of binding an enzyme inhibitor corresponding to said enzyme in a sample, the column being capable of delivering at least a part of the sample discharged from the column to the detector.
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Accused Products
Abstract
A process and device are disclosed to determine the activity of enzymes in liquids in a largely automatic manner. The device for carrying out this process has a column with an chromatographic carrier for treating a measurement sample. The carrier is mixed with a substance capable of binding to an enzyme inhibitor present in the measurement sample and that corresponds to at least one enzyme. A measurement sample supply is associated to one end of the column. A valve/pump arrangement for filling at least one test tube with a carrier and at least part of the measurement sample is connected downstream of the column, in the flow direction of the measurement sample. The carrier is dissociated into cleavage products by the action of the enzyme. The rise in concentration per unit of time of at least one of the cleavage products of the carrier is sensed during an incubation time. As an alternative or supplementary step, the enzyme that corresponds to at least one enzyme inhibitor is extracted by chromatography from a measurement sample to detect enzyme inhibitors in liquids and the thus treated measurement sample is tested for inhibitor concentration and/or activity.
13 Citations
52 Claims
- 1. A device for measuring the activity of an enzyme in liquid, the device comprising a flow through column and a detector, the column including a chromatographic carrier having a substance capable of binding an enzyme inhibitor corresponding to said enzyme in a sample, the column being capable of delivering at least a part of the sample discharged from the column to the detector.
- 14. A device for measuring the concentration of an enzyme inhibitor in liquids, the device comprising a flow through column and a detector, the column including a chromatographic carrier having a substance capable of binding an enzyme corresponding to said enzyme inhibitor in a sample, the column being capable of delivering at least a part of the sample discharged from the column to the detector.
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27. A method for measuring the activity of an enzyme in liquid comprising:
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applying a sample to a flow through column the column including a chromatographic carrier having a substance capable of binding an enzyme inhibitor corresponding to said enzyme in the sample;
delivering at least a part of the sample discharged from the column to a detector; and
measuring enzyme activity. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
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40. A method for measuring an enzyme inhibitor in liquid comprising:
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applying a sample to a flow through column, the column including a chromatographic carrier having a substance capable of binding an enzyme corresponding to said enzyme inhibitor in the sample;
delivering at least a part of the sample discharged from the column to a detector; and
measuring enzyme inhibitors. - View Dependent Claims (41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52)
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Specification