Funnel and one-piece column arrangement for retaining small quantities of chemical samples

• US 6,173,603 B1
• Filed: 09/03/1998
• Issued: 01/16/2001
• Est. Priority Date: 07/09/1996
• Status: Expired due to Fees

• Current Assignee: Amersham Pharmacia Biotech AB
• Original Assignee: Amersham Pharmacia Biotech AB
• Inventors: Horn, Marcus J.
• Primary Examiner(s): NOLAND, THOMAS
• Application Number:
  US09/146,507
• Time in Patent Office: 866 Days
• Field of Search: 73/615.5, 422/67, 422/70, 422/101, 422/102, 141/130
• US Class Current: 73/61.55
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/50255 Multi-well filtration
• CPC Class Codes: B01L 3/50855 using modular assemblies of strips or of individual wells
• CPC Class Codes: B01L 9/06 Test-tube stands; Test-tube holders
• CPC Class Codes: G01N 2030/027 Liquid chromatography
• CPC Class Codes: G01N 2030/8804 automated systems
• CPC Class Codes: G01N 2030/8831 involving peptides or proteins
• CPC Class Codes: G01N 2030/8881 Modular construction, specially adapted therefor
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 30/24 Automatic injection systems
• CPC Class Codes: G01N 30/36 in high pressure liquid systems
• CPC Class Codes: G01N 30/466 with separation columns in parallel
• CPC Class Codes: G01N 30/6043 in parallel
• CPC Class Codes: G01N 30/88 Integrated analysis systems specially adapted therefor, not covered by a single one of the groups G01N30/04 - G01N30/86
• CPC Class Codes: G01N 35/00732 Identification of carriers, materials or components in automatic analysers
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure

Abstract

A cassette is used with a chemical immobilization and treatment system. The system with the cassette performs various complex chemistries with minimal human intervention, near-zero dead volume, and flow-through protocols pursuant to a predetermined instruction set encoded on a multiple-address chemical treatment cassette assembly. The cassette assembly comprises a plurality of analyte sample columns (“mini-columns”), having a high-pressure interface capability to permit direct insertion of the mini-column into a high-pressure solvent line for use as a support column for HPLC analysis. A sample loader loads analyte samples either singly or simultaneously into the multiple, addressable mini-columns without the need to load the sample funnel/column assembly into a separate reaction chamber. The inventive system reads the machine readable instruction code and performs chemistries on the analyte, including mixing solvents, reactants and buffers; detaching and shuttling the analyte back and forth performing complex chemistries either on or off-column; solvation of powdered or lyophillized reagents; and performing in-line, on-board analysis. These protocols are performed automatically pursuant to instructions contained on a bar code, without having to remove the cassette or interface from the CTS, or otherwise require human intervention. The same chemistries may be performed on all sample mini-column addresses, or a separate completely independent set of protocols may be defined for each address, or for each block of addresses pursuant to the instructions contained in the machine readable code on the cassette.

Claims

1. A one-piece column for retaining a chemical sample, said column comprising a centrally disposed chamber extending longitudinally through a cylindrical column, said chamber terminating into a first open end, said first open end having an inlet port having a taper, said first open end enabling physical connection with a connection sleeve extending from a through-hole in the end of a sampling funnel to facilitate a leak-proof, high pressure seal with a nozzle interface, said connection resulting in transition from said funnel to the taper of said inlet port, said chamber terminating into a second open end, said second open end having:

an annular flange outwardly extending from the second open end and having an annular edge tapered inwardly so as to be accommodated by and self centering in an alignment hole of a supporting assembly having a complementary tapered bore for receiving the one-piece column, thereby providing alignment of the one-piece column within the supporting assembly;

an outlet port, said outlet port being in communication with said inlet port and tapered outwardly to facilitate a leak-proof, high pressure seal with a nozzle interface.

2. The one-piece column according to claim 1 further comprising a solid support material disposed in the centrally disposed chamber, said solid support material being treated to immobilize a chemical sample.

3. The one-piece column according to claim 2 wherein the centrally disposed chamber extending longitudinally through a cylindrical column includes solid support material disposed therein.

4. The one-piece column according to claim 3 wherein the solid material is retained in the column by porous frits.

5. The one-piece column according to claim 3 wherein the solid material is retained in the column by porous frits formed from one of sintered polyethylene, polypropylene, fluoropolymers, and glass.

6. The one-piece column according to claim 3 wherein the solid material includes silica that has been derivatized with a lipophyllic polymer, thus rendering the support hydrophobic.

7. The one-piece column according to claim 3 wherein the solid material includes one of polymer beads, resin beads, cellulose made to be hydrophillic.

8. The one-piece column according to claim 1 wherein said inlet taper permits application of a compression seal with respect to the one-piece column.

9. The one-piece column according to claim 1 wherein said taper of said inlet port is adapted for sealing engagement with a nozzle having a rounded tip.

10. The column according to claim 1 further comprising a solid support material disposed in the chamber, said solid support material being treated to immobilize a chemical sample.

11. The column according to claim 1 wherein said inlet taper permits application of a compression seal with respect to the column.

12. The column according to claim 1 wherein said taper of said inlet port is adapted for sealing engagement with a nozzle having a rounded tip.

13. A column for retaining a chemical sample, said column defining a chamber and having a first open end in turn having an inlet port having a taper, said first open end enabling physical connection with a connection sleeve extending from a through-hole in the end of a sampling funnel to facilitate a leak-proof, high pressure seal with a nozzle interface, said connection resulting in transition from said funnel to the taper of said inlet port, said chamber terminating into a second open end, said second open end having:

an annular flange outwardly extending from the second open end and having an annular edge tapered inwardly so as to be accommodated by and self centering in an alignment hole of a supporting assembly having a complementary tapered bore for receiving the one-piece column, thereby providing alignment of the one-piece column within the supporting assembly;

an outlet port, said outlet port being in communication with said inlet port and tapered outwardly to facilitate a leak-proof, high pressure seal with a nozzle interface.

14. The one-piece column according to claim 13 wherein the centrally disposed chamber extending longitudinally through a cylindrical column includes solid support material disposed therein.

15. The one-piece column according to claim 14 wherein the solid material is retained in the column by porous frits.

16. The one-piece column according to claim 14 wherein the solid material is retained in the column by porous frits formed from one of sintered polyethylene, polypropylene, fluoropolymers, and glass.

17. The one-piece column according to claim 14 wherein the solid material includes silica that has been derivatized with a lipophyllic polymer, thus rendering the support hydrophobic.

18. The one-piece column according to claim 14 wherein the solid material includes one of polymer beads, resin beads, cellulose made to be hydrophillic.

19. An arrangement for holding chemical samples, said arrangement including a funnel and a one-piece column for retaining a chemical sample, said column comprising a centrally disposed chamber having a first open end, said first open end having an inlet port having a taper, said first open end enabling physical connection with a connection sleeve extending from a through-hole in said funnel to facilitate a leak-proof, high pressure seal with a nozzle interface, said connection resulting in transition from said funnel to the taper of said inlet port, said chamber terminating in a second open end, said second open end having:

an annular flange outwardly extending from the second open end and having an annular edge tapered inwardly so as to be accommodated by and self centering in an alignment hole of a supporting assembly having a complementary tapered bore for receiving the one-piece column, thereby providing alignment of the one-piece column within the supporting assembly;

an outlet port, said outlet port being in communication with said inlet port and tapered outwardly to facilitate a leak-proof, high pressure seal with a nozzle interface.

20. The arrangement according to claim 19 further comprising a solid support material disposed in the centrally disposed chamber, said solid support material being treated to immobilize a chemical sample.

21. The arrangement according to claim 19 wherein said inlet taper permits application of a compression seal with respect to the one-piece column.

22. The arrangement according to claim 19 wherein said taper of said inlet port is adapted for sealing engagement with a nozzle having a rounded tip.