Biomatrix for soft tissue regeneration using mesenchymal stem cells

• US 6,174,333 B1
• Filed: 12/29/1998
• Issued: 01/16/2001
• Est. Priority Date: 06/06/1994
• Status: Expired due to Term

• Current Assignee: Case Western Reserve University, Osiris Therapeutics Incorporated (Smith & Nephew PLC)
• Original Assignee: Case Western Reserve University, Osiris Therapeutics Incorporated (Smith & Nephew PLC)
• Inventors: Fink, David J., Young, Randall G., Kadiyala, Sudhakar, Caplan, Arnold I.
• Primary Examiner(s): Isabella, David J.
• Application Number:
  US09/222,688
• Time in Patent Office: 749 Days
• Field of Search: 673/11, 673/12, 673/13, 673/14
• US Class Current: 623/11.11
• CPC Class Codes: A61B 17/06066 Needles, e.g. needle tip configurations making needles B21G1/00
• CPC Class Codes: A61B 17/06166 Sutures suture materials A61L17/00; manufacture of artificial threads D01D; treatment of threads D06M
• CPC Class Codes: A61B 17/1146 of tendons
• CPC Class Codes: A61B 2017/00004 (bio)absorbable, (bio)resorbable or resorptive

Abstract

An implant for repair of a tissue defect comprises a plurality of physiologically compatible load-bearing sutures for securing under tension tissue adjacent to the defect to be repaired, the sutures for supporting a tissue reparative cell mass in the defect and a tissue reparative cell mass supported thereby. The sutures have a central portion encapsulated in a cell containing matrix which is contracted under a tensile load by the cells thereof and formed into a mat sheet during the contraction. Spring metal wires hold the sutures in tension during the contraction. The matrix is a collagen gel or other material which the cells contract, the cells comprising human mesenchymal stem cells. The mat sheet is then rolled into a spiral roll with the sutures extending from opposite roll ends to form the desired implant. Different embodiments are disclosed.

Claims

1. An implant for repair of a tissue defect in an animal in need thereof, said implant comprising:

a contracted gel matrix formed in a planar mat having a length and a width forming a broad surface and a thickness relatively smaller than said width and length; and

mesenchymal stem cells within said contracted gel matrix, said cells having contracted said gel matrix in tension in a given direction.

2. The implant of claim 1 including at least one elongated load bearing support member extending in the given direction through and embedded within the matrix and having free opposing ends extending beyond the contracted matrix.

3. The implant of claim 1 wherein said cells are human mesenchymal stem cells (MSC).

4. The implant of claim 1 wherein the mat is rolled against itself and formed into a spiral roll configuration.

5. The implant of claim 2 wherein said at least one elongated load bearing support member includes a plurality of support members in spaced side by side relation in the mat, each member having adjacent free opposing ends.

6. The implant of claim 5 wherein the support members are biodegradable filaments.

7. The implant of claim 6 wherein the cells are human mesenchymal stem cells.

8. The implant of claim 7 wherein the mat is rolled against itself into a spiral configuration.

9. The implant of claim 2 wherein the at least one load bearing member is preloaded in tension along the given direction during contraction of the gel matrix.

10. The implant of claim 2 wherein the at least one load bearing member is selected from the group consisting of a single filament, multiple filaments, biodegradable fibrous materials, woven filaments, collagen fibers and at least one flexible, pliable filament.

11. An implant for repair of a tissue defect in an animal in need thereof, said implant comprising:

a contracted gel matrix formed in a mat sheet;

mesenchymal stem cells (MSCs) within said contracted gel matrix, said cells having contracted said gel matrix in tension in a given direction; and

a plurality of support members extending in the given direction through and embedded within the matrix, each member having opposing free ends extending beyond the contracted matrix.

12. The implant of claim 11 wherein the sheet is rolled about itself into a spiral so that the opposing free ends at each end are adjacent to each other.

13. The implant of claim 11 wherein the support members comprise biodegradable filaments.

14. The implant of claim 11 wherein the MSC cells are human mesenchymal stem cells.

15. An implant for repair of a tissue defect in an animal in need thereof, said implant comprising:

a contracted gel matrix formed as a flat mat; and

a plurality of load bearing support members lying encapsulated in the gel matrix, the support members having opposite end portions extending from the matrix, the support members extending in a given direction; and

mesenchymal stem cells within said contracted gel matrix, said matrix having been contracted by said cells in tension in said given direction.

16. The implant of claim 15 wherein the cells are human mesenchymal stem cells (MSC).

17. The implant of claim 15 wherein the contracted gel matrix and load bearing support members are rolled into a spiral terminating at first and second opposite ends, said load bearing support members extending from each said opposite ends.

18. An implant for repair of a tissue defect in an animal in need thereof, said implant comprising:

a contracted gel matrix formed as a flat mat and rolled against itself into a spiral;

at least one load bearing support member lying encapsulated in the gel matrix, the support member comprising a discrete support filament having opposite end portions extending from the matrix, the support filament extending in a given direction; and

mesenchymal stem cells within said contracted gel matrix, said matrix having been contracted by said cells in tension in said given direction.

19. The implant of claim 18 wherein the cells are human mesenchymal stem cells.

20. The implant of claim 18 including a plurality of said mats formed into said spiral roll in overlying contiguous relation.

21. The implant of claim 18 including a plurality of said contracted gel matrices disposed in parallel adjacent relationship to one another.