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Epoxide hydrolase inhibitor methods

  • US 6,174,695 B1
  • Filed: 05/14/1999
  • Issued: 01/16/2001
  • Est. Priority Date: 08/12/1997
  • Status: Expired due to Term
First Claim
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1. A method of screening for epoxide hydrolase inhibitors, comprising:

  • providing a cell culture under cell growth conditions, the cell culture expressing an epoxide hydrolase and having a determinable level of intracellular Ca2+;

    incubating the cell culture with a test compound and an oxylipin substrate; and

    determining a change in the level of intracellular Ca2+ correlating the change in the level of intracellular Ca2+ with the test compound being an epoxide hydrolase inhibitor.

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