Epoxide hydrolase inhibitor methods
First Claim
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1. A method of screening for epoxide hydrolase inhibitors, comprising:
- providing a cell culture under cell growth conditions, the cell culture expressing an epoxide hydrolase and having a determinable level of intracellular Ca2+;
incubating the cell culture with a test compound and an oxylipin substrate; and
determining a change in the level of intracellular Ca2+ correlating the change in the level of intracellular Ca2+ with the test compound being an epoxide hydrolase inhibitor.
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Abstract
The present invention provides methods of treating inflammatory diseases mediated by poly-unsaturated lipid metabolites by inhibiting epoxide hydrolase, methods for assaying or screening the epoxide hydrolase inhibitors for inhibitory specificity and for toxicity, and novel biologically active tetrahydrofuran diols of arachidonic acid, including antibodies thereto.
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Citations
6 Claims
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1. A method of screening for epoxide hydrolase inhibitors, comprising:
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providing a cell culture under cell growth conditions, the cell culture expressing an epoxide hydrolase and having a determinable level of intracellular Ca2+;
incubating the cell culture with a test compound and an oxylipin substrate; and
determining a change in the level of intracellular Ca2+ correlating the change in the level of intracellular Ca2+ with the test compound being an epoxide hydrolase inhibitor. - View Dependent Claims (2, 3, 4, 5, 6)
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Specification
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Current AssigneeRegents of the University of California (University of California)
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Original AssigneeRegents of the University of California (University of California)
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InventorsGrant, David F., Borhan, Babak, Moghaddam, Mehran F., Sisemore, Marlene F., Fergusson, James, Matoba, Kazuhiko, Cheek, Jeffrey M., Greene, Jessica F., Hammock, Bruce D., Zheng, Jiang
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Primary Examiner(s)GITOMER, RALPH J
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Application NumberUS09/312,207Time in Patent Office613 DaysField of Search435/18, 435/29, 435/69.2, 435/173.8, 435/252.3, 435/348US Class Current435/18CPC Class CodesC12Q 1/34 involving hydrolaseG01N 2500/10 involving cells
