Modified bioreactor

• US 6,174,720 B1
• Filed: 05/19/1999
• Issued: 01/16/2001
• Est. Priority Date: 09/19/1997
• Status: Expired due to Fees

• Current Assignee: Biotechna Environmental International Limited
• Original Assignee: Biotechna Environmental International Limited
• Inventors: Oxley, John, Startari, Joseph Francis
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/308,458
• Time in Patent Office: 608 Days
• Field of Search: 435/383, 435/420, 435/289.1, 435/292.1, 435/293.1, 435/293.2, 435/295.2
• US Class Current: 435/293.1
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 23/06 Tubular C12M23/08, C12M23/16 take precedence
• CPC Class Codes: C12M 41/00 Means for regulation, monitoring, measurement or control, e.g. flow regulation controlling or regulating chemical, physical or physicochemical processes B01J19/0006; heating or cooling apparatus for laboratory use B01L7/00; electro optical investigation of individual particles, flow cytometers G01N15/14; automatic analysis G01N35/00; controlling or regulating in general G06N

Abstract

Bioreactor apparatus for culture of living matter in a liquid medium includes a plurality of tubes connected at one end to a primary manifold section and at their other end to a secondary manifold section such that a flow of liquid containing the living matter can be established within the manifolds and tubes. The flow of liquid medium in such manifolds and/or tubes is capable of control by at least one gas injection device in communication with the primary manifold section and at least one gas injection device in communication with the secondary manifold section. Such an arrangement provides finer control of the flow conditions in the tubes forming part of the bioreactor and of the environmental conditions for culture of the living matter. The arrangement can have a relative simplicity of design which also permits a reduction in the total number of tanks and/or control loops usually associated with bioreactor apparatus of this type.

Claims

1. Bioreactor apparatus for culture of living matter in a liquid medium comprising a plurality of tubes connected at one end to a primary manifold section and at their other ends to a secondary manifold section such that a flow of liquid containing said living matter can be established within said manifolds and tubes, wherein the flow of liquid medium in said manifolds and/or said tubes is capable of control by at least one gas injection means in communication with the primary manifold section and at least one gas injection means in communication with the secondary manifold section.

2. Apparatus as claimed in claim 1 wherein each manifold section includes a header tank where gaseous media can disengage from liquid medium when flowing through said manifolds.

3. Apparatus as claimed in claim 1 wherein both primary and secondary manifold sections include at least two spaced apart upright tubular members, at least one of which is in liquid communication with said plurality of tubes.

4. Apparatus as claimed in claim 1 wherein the primary manifold section and the secondary manifold section each have at least one gas injection port in the vicinity of the lowermost region of a generally upright tubular component of said section.

5. Apparatus as claimed in claim 4 wherein each gas injection port is independently controllable as to gas used for injection and flow rates.

6. Apparatus as claimed in claim 1 wherein the primary manifold section and the secondary manifold section comprise, respectively, a U-shaped tubular arrangement and an inverted U-shaped tubular arrangement.

7. Apparatus as claimed in claim 1 wherein the primary and secondary manifold sections each comprise a pair of tubes, one located generally concentrically about the other.

9. A method of culturing living material including algae, bacteria or other microorganisms comprising:

providing the bioreactor apparatus of claims 1 or 8;

providing a source of said living material to said apparatus, and

operating said apparatus to culture said living material.

8. Bioreactor apparatus including at least two generally tubular coils wound helically to permit gaseous products to be carried out of the coils located one above the other or in grouped batches around an upstanding support structure, the coils having inlets to receive a flow of liquid medium within which biomass or other living material can be cultured and an outlet for said medium, the inlets of the coils arranged in liquid communication with a primary manifold and the outlets of the coils arranged in liquid communication with a secondary manifold, both said manifolds being in liquid communication whereby a flow of liquid medium can be provided from the primary to the secondary manifold, primary flow control means being provided to act upon liquid medium in or flowing from the primary manifold and secondary flow control means provided to act on liquid medium in or flowing into the secondary manifold, the primary and secondary flow control means being adjustable whereby flow of liquid medium through the coils can be varied by adjustment of either or both said flow control means.