Biosensor comprising a lipid membrane containing gated ion channels

• US 6,177,000 B1
• Filed: 03/13/2000
• Issued: 01/23/2001
• Est. Priority Date: 06/14/1997
• Status: Expired due to Fees

• Current Assignee: Coventry University, Sheet Pile, LLC
• Original Assignee: Coventry University
• Inventors: Peterson, Ian Robert
• Primary Examiner(s): Tung, T.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/445,817
• Time in Patent Office: 316 Days
• Field of Search: 204/403, 204/415, 204/418, 204/412, 205/778, 205/793, 205/775, 427/2.11, 427/2.13
• US Class Current: 205/780.5
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

A biosensor has a lipid membrane (7) containing gated ion channels sensitive to the presence or otherwise of an analyte molecule in a sample applied, in use, to a first side of the lipid membrane (7). The lipid membrane (7) is disposed between a pair of electrodes (1, 2) in which a first layer of porous gel (4) is applied to the first side of the lipid membrane (7).

Claims

1. A biosensor comprising a lipid membrane containing gated ion channels sensitive to the presence or otherwise of an analyte molecule in a sample applied, in use, to a first side of said lipid membrane, the lipid membrane being disposed between a pair of electrodes,

wherein the first side of the lipid membrane is coated with a first layer of porous get and the second side of the lipid membrane is coated with a second layer of porous gel.

2. A biosensor as claimed in claim 1, wherein the gel is a hydrogel.

3. A biosensor as claimed in claim 2 wherein the first layer of gel is such as to permit diffusion of biomolecules of approximately 1 kD to occur in less than 5 minutes.

4. A method of qualitatively or quantitatively determining an analyte molecule in a sample, which method comprises applying the sample to the first layer of porous gel in a biosensor according to claim 3.

5. A biosensor as claimed in claim 2 wherein the lipids of the lipid membrane have hydrocarbon tails with chain lengths Of C_12-22.

6. A method of qualitatively or quantitatively determining an analyte molecule in a sarnple, which method comprises applying the sample to the first layer of porous gel in a biosensor according to claim 2.

7. A biosensor as claimed in claim 1 wherein the first layer of gel is such as to permit diffusion of biomolecules of approximately 1 kD to occur in less than 5 minutes.

8. A biosensor as claimed in claim 7 wherein the lipids of the lipid membrane have hydrocarbon tails with chain lengths of C_12-22.

9. A method of qualitatively or quantitatively determining an analyte molecule in a sample, which method comprises applying the sample to the first layer of porous gel in a biosensor according to claim 7.

10. A biosensor as claimed in claim 1 wherein the lipids of the lipid membrane have hydrocarbon tails with chain lengths of C_12-22.

11. A method of qualitatively or quantitatively determining an analyte molecule in a sample, which method comprises applying the sample to the first layer of porous gel in a biosensor according to claim 1.

12. A method of assembling a biosensor comprising coating each of two planar electrodes with a layer of porous gel, placing lipid solution on to one of said layers and then placing one electrode on top of the other such that the gel layers are disposed between them and are separated by a lipid membrane.

13. A method as claimed in claim 12 wherein prior to the formation of the lipid membrane the membrane-forming lipid and the molecules defining gated ion channels are dissolved in a solvent.

14. A method as claimed in claim 13 wherein the solvent is chloroform.

15. A method as claimed in claim 12 wherein, prior to the formation of the lipid membrane the membrane-forming lipid and molecules defining gated ion channels are incorporated into an inverted emulsion with an alkane-rich continuous phase.

16. A method as claimed in claim 15 wherein the alkane is hexane.