×

Simple immunochemical semi-quantitative assay method and apparatus

  • US 6,177,281 B1
  • Filed: 06/11/1996
  • Issued: 01/23/2001
  • Est. Priority Date: 06/02/1993
  • Status: Expired due to Fees
First Claim
Patent Images

1. An apparatus for an immunochemical semi-quantitative assay according to immunochromatography, the apparatus comprising a plurality of units;

  • each unit comprisingan antibody-fixed portion (B) where an antibody for trapping an amount of an analyte in a sample is present and fixed in a predetermined amount, a labeled substance presence portion (C) where a labeled substance as an index for detecting the presence of the analyte is present in a chromatographically mobile state, and a detection portion (D) where a detecting substance for detecting the labeled substance is present and fixed;

    the apparatus comprising a plurality of said units, a sample addition portion (A) for adding the sample containing the analyte, the sample addition portion (A) being common to each unit, and an absorption portion (E) for removing the labeled substance which does not participate in the detection of the analyte, together with the added sample, by absorption, the absorption portion (E) being either common to each unit or independent for each unit;

    wherein the amount of the trapping antibody fixed in the antibody-fixed portion (B) of each unit differs from one unit to another and the amounts of the trapping antibodies are consecutively varied stepwise, and wherein the analyte is a hapten, the antibody for trapping the analyte, fixed in the antibody-fixed portion (B), is a hapten trapping antibody, the labeled substance is a hapten labeled antibody which binds to the analyte, and the detecting substance binds to the labeled hapten antibody competitively with the hapten which is the analyte.

View all claims
  • 1 Assignment
Timeline View
Assignment View
    ×
    ×