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Nucleic acid mediated electron transfer

  • US 6,180,352 B1
  • Filed: 12/10/1999
  • Issued: 01/30/2001
  • Est. Priority Date: 12/10/1993
  • Status: Expired due to Term
First Claim
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1. A method of detecting the presence of a sequence in a nucleic acid sample, said method comprising:

  • a) applying an input signal to an electrode comprising a hybridization complex, wherein said hybridization complex comprises;

    1) a single stranded nucleic acid;

    2) a target sequence hybridized to said single stranded nucleic acid; and

    3) at least one covalently attached electron donor moiety;

    such that an electron is transferred from said electron donor moiety to said electrode such that the redox state of said electron donor moiety is changed to form an electron acceptor moiety;

    b) transferring an electron from a reductant to said electron acceptor moiety; and

    c) detecting electron transfer between said electron donor moiety and said electrode, wherein the presence of electron transfer is an indication of the presence of said target sequence.

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