Infrared spectroscopy for medical imaging

• US 6,181,414 B1
• Filed: 08/05/1999
• Issued: 01/30/2001
• Est. Priority Date: 02/06/1998
• Status: Expired due to Fees

• Current Assignee: Morphometrix Technologies Incorporated
• Original Assignee: Morphometrix Technologies Incorporated
• Inventors: Rosenblatt, Gordon, Maclean, Daniel S., Raz, Ryan S.
• Primary Examiner(s): Evans, F. L.
• Application Number:
  US09/368,521
• Time in Patent Office: 544 Days
• Field of Search: 356/51, 250/339.05, 348/65, 348/79, 348/166, 348/168
• US Class Current: 356/51
• CPC Class Codes: G01N 15/1433 using image recognition
• CPC Class Codes: G01N 2021/3133 with selection of wavelengths before the sample
• CPC Class Codes: G01N 2021/3137 with selection of wavelengths after the sample
• CPC Class Codes: G01N 2021/3148 using three or more wavelengths
• CPC Class Codes: G01N 2021/3155 Measuring in two spectral ranges, e.g. UV and visible
• CPC Class Codes: G01N 21/3151 using two sources of radiation of different wavelengths G01N21/33 - G01N21/39 take precedence
• CPC Class Codes: G01N 21/3563 for analysing solids; Preparation of samples therefor

Abstract

A multi-channel spectral imaging module for an automated testing system for cytological applications. The multi-channel spetral imaging module generates four channels of digitized images of a cytological specimen for further processing in the automated testing system. The multi-spectral imaging module comprises a visible light source and an infrared light source, an optical imaging module, a prism module, and an output stage. The cytological specimen is strobed by the visible light and infrared light sources and the illuminated images of the specimen are focussed and coupled to the prism module by the optical imaging module. The prism module breaks the illuminated image into four channels comprising three visible light channels and an infrared channel. The output stage comprises a CCD array sensor for each channel. The CCD array sensor digitizes the image for channel and produces an output for further processing. The digitized output from the infrared channel provides additional information which is used by the automated testing system. The additional information derived from the infrared channel includes segmentation information, new identifying features for the specimen, and discrimination measures independent of the visible light channels. In another embodiment, a fifth channel is provided for a reference infrared output and the two infrared channels are operated in a differential mode.

Claims

1. A multi-channel spectral imaging module for an automated testing system for cytological application, said multi-channel spectral imaging module comprising:

(a) a light source module for illuminating a cytological specimen, said light source including a visible light source and an infrared light source and a control input for actuating said visible and infrared light sources;

(b) an optical imaging module for receiving illuminated images of said cytological specimen and including a lens system for focussing said illuminated images;

(c) a prism module for receiving said illuminated images from said optical imaging module, said prism module including three prism elements for producing three respective image channels in the visible spectrum and a fourth prism element for producing an image channel in the infrared spectrum, and said prism module further including a charge coupled device for each of said channels for converting the respective image into an electrical signal.

2. The multi-channel spectral imaging module as claimed in claim 1, wherein said prism module includes means for producing a second infrared output channel, said first and second infrared output channels providing a differential channel pair wherein said first infrared channel comprises an interrogation signal and said second infrared channel comprises a reference signal.

3. The multi-spectral imaging module as claimed in claim 1, wherein said image channels in the visible spectrum comprise a first optical band centered at 530 nanometers, a second optical band centered at 630 nanometers, and a third optical band centered at 577 nanometers, and said infrared spectral band comprises a 1215 cm⁻

1 band.

4. The multi-channel spectral imaging module as claimed in claim 2, wherein said interrogation signal comprises a 1215 cm⁻

1 band and said reference band comprises a 1240 cm<sup>−

1</sup> band.

5. The multi-channel spectral imaging module as claimed in claim 1, wherein said prism module includes means for producing a second infrared output channel, said first and second infrared output channels providing information pertaining to features associated with said cytological specimen.

6. The multi-channel spectral imaging module as claimed in claim 5, wherein said information corresponds to an independent discriminate measure for said cytological specimen.

7. The multi-channel spectral imaging module as claimed in claim 5, wherein said information provides a statistical measurement associated with nuclear regions in said cytological specimen.

8. A method for generating multi-spectral image data for a cytological specimen, said method comprising the steps of:

(a) exposing said cytological specimen to a burst of broad-band light to produce a broad-band light image;

(b) separating said broad-band light image into a plurality of spectral bands;

(c) simultaneously capturing an image for each of said spectral bands and generating electrical signals corresponding to each of said captured spectral images;

(d) converting the electrical signals corresponding to said captured spectral images into a data form suitable for further processing by an automated testing system;

(e) exposing said cytological specimen to a burst of light in an infrared spectral band;

(f) simultaneously capturing an image in said infrared spectral band and generating an electrical signal corresponding to said captured infrared spectral image;

(g) converting the electrical signal corresponding to said captured infrared spectral image into a data form suitable for further processing by an automated testing system.

9. A method for generating multi-spectral image data for a cytological specimen, said method comprising the steps of:

(a) exposing said cytological specimen to a burst of broad-band light to produce a broad-band light image;

(b) separating said broad-band light image into a plurality of spectral bands;

(c) simultaneously capturing an image for each of said spectral bands and generating electrical signals corresponding to each of said captured spectral images;

(d) converting the electrical signals corresponding to said captured spectral images into a data form suitable for further processing by an automated testing system;

(e) exposing said cytological specimen to a burst of infrared light to produce an infrared light image;

(f) separating said infrared light image into a plurality of infrared spectral bands;

(g) simultaneously capturing an image for each of said infrared spectral bands and generating electrical signals corresponding to each of said captured infrared spectral images;

(h) converting the electrical signals corresponding to said captured infrared spectral images into a data form suitable for further processing by an automated testing system.

10. The method for generating multi-spectral image data for a cytological specimen as claimed in claim 9, wherein said infrared spectral bands comprise a first infrared spectral band and a second infrared spectral band, said first and second infrared spectral bands providing a differential channel wherein said first infrared spectral band provides an interrogation channel and said second infrared spectral band provides a reference channel.

11. The method for generating multi-spectral image data for a cytological specimen as claimed in claim 9, wherein said spectral bands comprise a first optical band centered at 530 nanometers, a second optical band centered at 630 nanometers, and a third optical band centered at 577 nanometers, and said infrared spectral band comprises a 1215 cm⁻

1 band.

12. The method for generating multi-spectral image data for a cytological specimen as claimed in claim 10, wherein said first infrared spectral band comprises a 1215 cm⁻

1 band and said second infrared spectral band comprises a 1240 cm<sup>−

1</sup> band.