Process and measuring system for detection of substances emitted or perspired through the skin

• US 6,183,418 B1
• Filed: 01/30/1998
• Issued: 02/06/2001
• Est. Priority Date: 05/06/1996
• Status: Expired due to Fees

• Current Assignee: Trace Biotech AG
• Original Assignee: META Mebtechnische Systeme GmbH, Moller Feinmechanik GmbH & Company, Trace Analysensysteme GmbH
• Inventors: Kuennecke, Wolfgang
• Primary Examiner(s): O'Connor, Cary
• Assistant Examiner(s): ASTORINO, MICHAEL C
• Application Number:
  US09/015,911
• Time in Patent Office: 1,103 Days
• Field of Search: 600/363-364, 600/358
• US Class Current: 600/363
• CPC Class Codes: G01N 13/00 Investigating surface or boundary effects, e.g. wetting power; Investigating diffusion effects; Analysing materials by determining surface, boundary, or diffusion effects scanning-probe techniques or apparatus G01Q
• CPC Class Codes: G01N 2001/4016 being a selective membrane, e.g. dialysis or osmosis
• CPC Class Codes: G01N 33/146 containing alcohol
• CPC Class Codes: G01N 33/4972 Determining alcohol content for vehicle safety devices B60K28/06
• CPC Class Codes: G01N 35/08 using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis

Abstract

The process for detection and for quantitative determination of substances emitted or perspired through the skin is derived from flow diffusion analysis. The measuring system conceived for this purpose uses a diffusion half cell through which an acceptor medium flows and which is closed by a membrane. For the duration of the measurement, the membrane is brought into contact with the skin or a closed gas volume formed over the skin. With the process and the related measuring system, the blood alcohol level can be determined with a good degree of precision indirectly via the quantity of (gaseous) ethanol emitted through the skin.

Claims

1. A process for detection of a substance emitted or perspired through the skin by means of a detection system responding to samples of the substance, the process using flow diffusion analysis and comprising the steps of:

flowing an acceptor medium to a diffusion half cell which is closed with a membrane, the membrane being adapted to be placed onto the skin of a person to be examined or brought into contact with a closed gas volume formed over the skin;

bringing into contact the substance to be detected and the membrane;

and

feeding the acceptor medium laded with the substance to be detected to the detection system for detection of the substance.

2. The process according to claim 1, further comprising maintaining the acceptor medium in the diffusion half cell for a period of time in order to bring about a defined enrichment of the substance to be detected in the acceptor medium.

3. The process according to claim 1, further comprising adding a detection reagent to the acceptor medium for determination of the substance and a detection reaction detected at the detection system.

4. The process according to claim 3, wherein a biosensor is used as the detection system.

5. The process according to claim 4, wherein the biosensor detects alcohol using alcohol oxidase (AOD) with amperometric determination of equimolecular-formed hydrogen peroxide using a platinum thick-layer electrode.

6. The process according to claim 4, further comprising calibrating the detection system before detecting the substance.

7. The process according to claim 1, wherein the substance to be detected is alcohol.

8. The process according to claim 1, further comprising providing a replaceable measuring head for housing the membrane and adapted for placing onto the skin of the person to be examined or brought into contact with the closed gas volume formed over the skin.

9. The process according to claim 1, wherein the substance to be detected permeates through the membrane and combines with the acceptor medium prior to the feeding step.

10. The process according to claim 1, further comprising detecting the substance by one of electrochemical, amperometrical and optical detection.

11. The process according to claim 1, further comprising controlling the flow of the acceptor medium.

12. The process according to claim 11, wherein the controlling of the flow of the acceptor medium includes increasing or decreasing the flow of the acceptor medium to the diffusion half cell.

13. The process according to claim 1, wherein the detecting of the substance includes measuring the substance.