Method and apparatus for detecting viruses using primary and secondary biomarkers

• US 6,183,950 B1
• Filed: 08/04/1998
• Issued: 02/06/2001
• Est. Priority Date: 07/31/1998
• Status: Expired due to Term

• Current Assignee: Colorado School of Mines
• Original Assignee: Colorado School of Mines
• Inventors: Madonna, Angelo J., Voorhees, Kent J.
• Primary Examiner(s): Wortman, Donna C.
• Assistant Examiner(s): Brumback, Brenda G.
• Application Number:
  US09/130,207
• Time in Patent Office: 917 Days
• Field of Search: 435/5, 436/71
• US Class Current: 435/5
• CPC Class Codes: H01J 49/04 Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components

Abstract

The present invention relates to the detection of the likely presence of a virus in the environment. The detection is accomplished in a relatively rapid fashion that permits countermeasures to be taken to reduce the debilitating or deadly effects of the virus upon the target population. In one embodiment, the detection is accomplished by looking for the mass spectral signature or biomarker for a lipid, which is present in the cell cultures used to produce the virus. One biomarker that is considered particularly diagnostic for the presence of a virus is cholesterol.

Claims

1. A method for detecting the likely presence of a virus in the environment so that countermeasures can be deployed, the method comprising:

sampling the atmosphere;

analyzing the sampled atmosphere to determine if cholesterol, which is indicative of a virus, is present, wherein said step of analyzing includes subjecting the sampled atmosphere to pyrolysis to free any cholesterol present in the sampled atmosphere; and

issuing, if a cholesterol is present, an alarm so that countermeasures can be deployed against the virus.

2. A method, as claimed in claim 1, wherein:

said step of sampling comprises using a laser technique to assess the presence or absence of an aerosol in the atmosphere.

3. A method, as claimed in claim 1, wherein said step of analyzing comprises:

obtaining a mass spectrum for the sample; and

inspecting said mass spectrum above about 200 m/z for peaks indicative of the presence of cholesterol.

4. A method, as claimed in claim 1, wherein:

said step of analyzing comprises using gas chromatography.

5. A method, as claimed in claim 1, wherein:

said step of issuing comprises directing an individual to a particular location.

6. A method, as claimed in claim 1, wherein:

said steps of sampling and analyzing cumulatively take less that about 15 minutes.

7. A method, as claimed in claim 1, wherein:

said steps of sampling and analyzing cumulatively take no more than about 5 minutes.

8. A method, as claimed in claim 1, wherein:

said step of analyzing comprises detecting cholesterol as a primary biomarker or a secondary biomarker.

9. A method, as claimed in claim 1, wherein:

said step of sampling comprises using light scattering to assess the presence or absence of an aerosol in the atmosphere.

10. A method, as claimed in claim 1, wherein:

said step of analyzing comprises using liquid chromatography.

11. A method, as claimed claim 1, wherein:

said step of analyzing comprises using Fourier Transform Infrared Spectroscopy.

12. A method, as claimed in claim 1, wherein:

said step of analyzing comprises using colorimetry.