Cellulases, the genes encoding them and uses thereof
First Claim
Patent Images
1. A nucleic acid molecule encoding a polypeptide having the enzymatic activity of a cellulase, selected from the group consisting of:
- (a) nucleic acid molecules encoding a polypeptide comprising the amino acid sequence as depicted in FIGS. 19 (SEQ ID NO;
17) or 21 (SEQ ID NO;
33);
(b) nucleic acid molecules encoding a polypeptide comprising the amino acid sequence as depicted in FIGS. 23 (SEQ ID NO;
35) or 27 (SEQ ID NO;
37);
(c) nucleic acid molecules comprising the coding sequence of the nucleotide sequence as depicted in FIGS. 19 (SEQ ID NO;
30) or 21 (SEQ ID NO;
32);
(d) nucleic acid molecules comprising the coding sequence of the nucleotide sequence as depicted in FIGS. 23 (SEQ ID NO;
34) or 27 (SEQ ID NO;
36);
(e) nucleic acid molecules encoding a.polypeptide comprising the amino acid sequence encoded by the DNA insert contained in DSM 11024, DSM 11012, DSM 11025 or DSM 11014;
(f) nucleic acid molecules encoding a polypeptide comprising the amino acid sequence encoded by the DNA insert contained in DSM 11026, DSM 11011, DSM 11013 or DSM 11027;
(g) nucleic acid molecules comprising the coding sequence of the DNA insert contained in DSM 11024, DSM 11012, DSM 11025 or DSM 11014;
(h) nucleic acid molecules comprising the coding sequence of the DNA insert contained in DSM 11026, DSM 11011, DSM 11013 or DSM 11027;
(i) nucleic acid molecules hybridizing under stringent conditions to a molecule of any one of (a), (c), (e) or (g);
(j) nucleic acid molecules the coding sequence of which differs from the coding sequence of a nucleic acid molecule of any one of (a) to (i) due to the degeneracy of the genetic code; and
(k) nucleic acid molecules encoding a polypeptide having cellulase activity and having an amino acid sequence which shows at least 80% identity to a sequence as depicted in FIGS. 19 (SEQ ID NO;
31), 21 (SEQ ID NO;
33), 23 (SEQ ID NO;
35), or 27 (SEQ ID NO;
37).
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Abstract
Genes encoding novel cellulases, and a gene encoding a protein that facilitates the action of such novel cellulases, the novel cellulases and a protein that facilitates the action of such cellulases, and enzyme preparations containing such proteins are described The native hosts and the culture medium of said hosts containing said novel cellulases are also disclosed. These proteins are especially useful in the textile and detergent industry and in pulp and paper industry.
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Citations
44 Claims
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1. A nucleic acid molecule encoding a polypeptide having the enzymatic activity of a cellulase, selected from the group consisting of:
-
(a) nucleic acid molecules encoding a polypeptide comprising the amino acid sequence as depicted in FIGS. 19 (SEQ ID NO;
17) or 21 (SEQ ID NO;
33);
(b) nucleic acid molecules encoding a polypeptide comprising the amino acid sequence as depicted in FIGS. 23 (SEQ ID NO;
35) or 27 (SEQ ID NO;
37);
(c) nucleic acid molecules comprising the coding sequence of the nucleotide sequence as depicted in FIGS. 19 (SEQ ID NO;
30) or 21 (SEQ ID NO;
32);
(d) nucleic acid molecules comprising the coding sequence of the nucleotide sequence as depicted in FIGS. 23 (SEQ ID NO;
34) or 27 (SEQ ID NO;
36);
(e) nucleic acid molecules encoding a.polypeptide comprising the amino acid sequence encoded by the DNA insert contained in DSM 11024, DSM 11012, DSM 11025 or DSM 11014;
(f) nucleic acid molecules encoding a polypeptide comprising the amino acid sequence encoded by the DNA insert contained in DSM 11026, DSM 11011, DSM 11013 or DSM 11027;
(g) nucleic acid molecules comprising the coding sequence of the DNA insert contained in DSM 11024, DSM 11012, DSM 11025 or DSM 11014;
(h) nucleic acid molecules comprising the coding sequence of the DNA insert contained in DSM 11026, DSM 11011, DSM 11013 or DSM 11027;
(i) nucleic acid molecules hybridizing under stringent conditions to a molecule of any one of (a), (c), (e) or (g);
(j) nucleic acid molecules the coding sequence of which differs from the coding sequence of a nucleic acid molecule of any one of (a) to (i) due to the degeneracy of the genetic code; and
(k) nucleic acid molecules encoding a polypeptide having cellulase activity and having an amino acid sequence which shows at least 80% identity to a sequence as depicted in FIGS. 19 (SEQ ID NO;
31), 21 (SEQ ID NO;
33), 23 (SEQ ID NO;
35), or 27 (SEQ ID NO;
37).- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44)
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Specification
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Current AssigneeAB Enzymes Oy (Garfield Weston Foundation)
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Original AssigneeRohm Enzyme Finland Oy
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InventorsPaloheimo, Marja, Lantto, Raija, Vehmaanperä, Jari, Suominen, Pirkko, Miettinen-Oinonen, Arja, Haakana, Heli, Elovainio, Minna, Joutsjoki, Vesa, Londesborough, John, Mäntylä, Arja
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Primary Examiner(s)Patterson, Jr., Charles L.
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Application NumberUS09/329,350Time in Patent Office607 DaysField of Search435/209, 435/320.1, 435/254.3, 435/254.6, 536/23.2, 536/24.3US Class Current435/209CPC Class CodesC11D 3/38645 containing cellulaseC12N 9/2437 Cellulases (3.2.1.4; 3.2.1....C12Y 302/01004 Cellulase (3.2.1.4), i.e. e...C12Y 302/01091 Cellulose 1,4-beta-cellobio...
