Polymerized crystalline colloidal arrays

• US 6,187,599 B1
• Filed: 07/07/1998
• Issued: 02/13/2001
• Est. Priority Date: 11/06/1996
• Status: Expired due to Term

• Current Assignee: University Of Pittsburgh (University of Pittsburgh of The Commonwealth System of Higher Education)
• Original Assignee: University Of Pittsburgh (University of Pittsburgh of The Commonwealth System of Higher Education)
• Inventors: Holtz, John H., Asher, Sanford A.
• Primary Examiner(s): CEPERLEY, MARY
• Application Number:
  US09/111,610
• Time in Patent Office: 952 Days
• Field of Search: 436/531, 436/73, 436/518, 436/529, 436/79, 252/582, 359/885, 435/14
• US Class Current: 436/531
• CPC Class Codes: B01J 13/00 Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
• CPC Class Codes: B01J 13/0065 containing an organic phase
• CPC Class Codes: G01J 3/18 using diffraction elements, e.g. grating gratings per se G02B
• CPC Class Codes: G01N 2021/772 Tip coated light guide
• CPC Class Codes: G01N 2021/7723 Swelling part, also for adsorption sensor, i.e. without chemical reaction
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 31/222 for investigating moisture content
• CPC Class Codes: G02B 26/002 the movement or the deformation controlling the frequency of light, e.g. by Doppler effect
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/811 Test for named disease, body condition or organ function
• CPC Class Codes: Y10T 436/144444 Glucose
• CPC Class Codes: Y10T 436/204998 Inorganic carbon compounds
• CPC Class Codes: Y10T 436/205831 Carbon monoxide only
• CPC Class Codes: Y10T 436/207497 Molecular oxygen
• CPC Class Codes: Y10T 436/209163 Dissolved or trace oxygen or oxygen content of a sealed environment

Abstract

Novel sensor devices composed of a crystalline colloidal array (CCA) polymerized in a hydrogel are disclosed. The hydrogels are characterized as being capable of shrinking and swelling in response to specific stimuli applied thereto. As the hydrogels shrink or swell, the lattice structure of the CCA embedded therein changes, thereby changing the wavelength of light diffracted by the CCA. Thus by monitoring the change in diffracted wavelength, the concentration of a stimulus is determined. The gels can be modified to sense numerous different stimuli. The sensor devices are specific in that they are modified to react with only one species or family of species. These sensors have various applications in areas including, for example, environmental and chemical systems, chemomechanical systems, sensor devices and medical diagnostic tools. Various methods for making and using these devices are also disclosed.

Claims

1. A composition comprising a polymerized hydrogel that undergoes a volume change in response to a specific chemical stimulus, and a crystalline colloidal array of charged particles having a lattice spacing, the array embedded in the hydrogel, wherein the hydrogel includes a chemical stimulus recognition component capable of recognizing the specific chemical stimulus, which component is selected from the group consisting of crown ethers, cyclodextrins, calixarenes, biomolecular recognition components, and chelating agents;

and further wherein the crystalline colloidal array has light diffracting properties and the volume change causes the lattice spacing to change thereby causing the light diffracting properties to change.

2. The composition according to claim 1 wherein said hydrogel is comprised of a first comonomer that is a gel monomer, a second comonomer, comprising the chemical stimulus recognition component, and a cross-linking agent.

3. The composition according to claim 1 wherein said hydrogel is hydrophilic.

4. The composition according to claim 2 wherein said gel monomer is ion-free.

5. The composition according to claim 4 wherein said gel monomer is selected from the group consisting of acrylamide, purified agarose, N-vinylpyrolidine, and methacrylate.

6. The composition according to claim 4 wherein said gel monomer is N-isopropylacrylamide.

7. The composition according to claim 2 wherein the cross-linking agent is selected from the group consisting of N,N′

-methylenebisacrylamide, methylenebis methacrylamide and ethyleneglycol-dimethacrylate.

8. The composition according to claim 2 wherein the hydrogel further comprises a third monomer.

9. The composition according to claim 8 wherein the third monomer is selected from the group consisting of acrylamide and substituted acrylamide.

10. The composition according to claim 1 wherein the chemical stimulus recognition component is selected from the group consisting of crown ethers, cyclodextrins and calixarenes.

11. The composition according to claim 1 wherein said chemical stimulus recognition component is 4-acylamidobenzo 18-crown-6 ether.

12. The composition according to claim 1 wherein the chemical stimulus recognition component is a biomolecular recognition component.

13. The composition according to claim 12 wherein said biomolecular recognition component is selected from the group consisting of enzymes, antigens, nucleic acids, nucleic acid sequences, amino acids, amino acid sequences, peptides, porphyrins, ferritins, pheromone receptors, and antibodies.

14. The composition according to claim 12 wherein the hydrogel is further comprised of a gel monomer component and one or more linking molecules, the linking molecules link said biomolecular recognition component to said gel monomer component.

15. The composition according to claim 14 wherein said linking molecule is 5-(biotinamido)pentylamine.

16. The composition according to claim 14 wherein said linking molecule is bound to a second linking molecule or to the gel monomer component.

17. The composition according to claim 16 wherein said linking molecule is avidin.

18. The composition according to claim 1 wherein the stimulus recognition component is a chelating agent.

19. The composition according to claim 1 wherein the volume change is between about 0.1 and 50%.

20. The composition according to claim 1 wherein the crystalline colloidal array is comprised of particles selected from the group consisting of colloidal polystyrene, polymethylmethacrylate, silicon dioxide, aluminum oxide, poly tetrafluoroethylene and poly N-isopropylacrylamide.

21. The composition according to claim 1 wherein the chemical stimulus is selected from the group consisting of lead ions, potassium ions and sodium ions.

22. The composition according to claim 1 wherein the hydrogel comprises more than one polymer network, the polymer networks interpenetrating each other.

23. The composition according to claim 22 wherein one of the polymer networks comprises the stimulus recognition component.

24. The composition according to claim 23 wherein said stimulus recognition component is selected from the group consisting of crown ethers, cyclodextrins, and calixarenes.

25. The composition according to claim 24 wherein said stimulus recognition component is 4-acrylamidobenzo 18-crown-6 ether.

26. The composition according to claim 22 wherein the array is comprised of particles selected from the group consisting of colloidal polystyrene, polymethacrylate, silicon dioxide, aluminum oxide, polytetrafluoroethylene and poly N-isopropylacrylamide.

27. The composition according to claim 26 wherein at least one of said polymer networks is comprised of a gel monomer, the gel monomer is selected from the group consisting of acrylamide, purified agarose, N-vinyl pyrolidine and methacrylate.

28. The composition according to claim 22 wherein the hydrogel is hydrophilic.

29. The composition according to claim 22 wherein at least one of said polymer networks is comprised of gel monomer, the gel monomer being ion-free.

30. The composition according to claim 29 wherein the gel monomer is N-isopropylacrylamide.

31. The composition according to claims 22 wherein the volume change in response to the specific chemical stimulus is between about 0.1% and 50%.

32. A composition comprising a polymerized hydrogel that undergoes a volume change in response to a specific chemical stimulus, a crystalline colloidal array of charged particles having a lattice spacing, which array is embedded in the hydrogel, and a material, embedded within said hydrogel, which is released by said change in volume;

wherein the crystalline colloidal array has light diffracting properties and the volume change causes the lattice spacing to change thereby causing the light diffracting properties to change.

33. A composition comprising a polymerized hydrogel that undergoes a volume change in response to a specific chemical stimulus, and a crystalline colloidal array of charged particles having a lattice spacing, which array is embedded in the hydrogel, wherein the chemical stimulus is selected from the group consisting of lead ions, potassium ions, and sodium ions;

wherein the crystalline colloidal array has light diffracting properties and the volume change causes the lattice spacing to change thereby causing the light diffracting properties to change.