Method of mass-analyzing body fluid and apparatus therefor

• US 6,190,316 B1
• Filed: 03/22/1999
• Issued: 02/20/2001
• Est. Priority Date: 03/25/1998
• Status: Expired due to Term

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Hirabayashi, Atsumu, Koizumi, Hideaki, Hirabayashi, Yukiko
• Primary Examiner(s): WINAKUR, ERIC FRANK
• Application Number:
  US09/273,554
• Time in Patent Office: 701 Days
• Field of Search: 600/309, 600/366, 250/281, 250/282, 250/288, 422/68.1
• US Class Current: 600/309
• CPC Class Codes: A61B 2010/0077 Cerebrospinal fluid
• CPC Class Codes: A61B 5/145 Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissuemeasuring of blood pressure or blood flow A61B5/02; non-radiation detecting or locating of foreign bodies in blood A61B5/06
• CPC Class Codes: A61B 5/14528 invasively
• CPC Class Codes: A61B 5/14557 specially adapted to extracorporeal circuits
• CPC Class Codes: A61B 5/1459 invasive, e.g. introduced into the body by a catheter A61B5/1464 takes precedence
• CPC Class Codes: A61B 5/4064 Evaluating the brain A61B5/031, A61B5/369, A61B5/14553 take precedence
• CPC Class Codes: H01J 49/0431 for liquid samples interfaces to liquid chromatographs G01N30/7233

Abstract

A living body fluid analyzing system includes a microdialysis for sending a first solution having an osmotic pressure which is substantially similar to a osmotic pressure of a body fluid into a living body and extracting a second solution from the living body. A first flow passage is provided in which the second solution from the microdialysis flows and a second flow passage is provided which mixes the second solution with an organic solution. Furthermore, there is provided a gas source and a gas flow controller which controls a flow quantity of the gas from the gas source and a third flow passage in which a gas introduced from the gas source flows. An ion source is provided having an orifice for spraying and ionizing the second solution from the second flow passage at an end of the third flow passage, and a mass spectrometer is provided for mass-analyzing the ions sprayed from the orifice.

Claims

1. A mass-analyzing apparatus for a living body fluid, comprising:

a microdialysis probe for collecting said living body fluid from a living body,

a first flow passage through which an analyte solution including the living body fluid collected by the microdialysis probe flows,

a second flow passage having a branched portion connected with an end portion of said first flow passage, said branched portion mixing the analyte solution with an organic solvent,

a third flow passage through which the analyte solution mixed in said second flow passage flows,

a fourth flow passage for causing a gas to flow around said third flowing passage,

an ion source for spraying and ionizing the analyte solution from said third flow passage by a high speed gas from said fourth flow passage so as to provide ionized ions, and

an analyzing unit for mass-analyzing the ionized ions in real time.

2. The mass-analyzing apparatus for a living body fluid as claimed in claim 1, further comprising an electrode provided in proximity to said third flow passage included in said ion source, wherein a voltage is supplied between said electrode and said analyte solution and thereby an electric field is applied to said analyte solution.

3. A mass-analyzing apparatus as claimed in claim 1, wherein said microdialysis probe collects living body fluid from said living body by sending a solution having an osmotic pressure which is substantially similar to an osmotic pressure of said living body fluid into said living body and collects said living body fluid from said living body as part of said analyte solution which flows through said first flow passage.

4. A mass-analyzing apparatus for a living body fluid, comprising:

a first flow passage for flowing a sample solution containing a sample to be analyzed,

a second flow passage for supplying an organic solution for moving with said sample to be analyzed flowing on said first flow passage,

a third flow passage for introducing said solutions from said first flow passage and said second flow passage into an ion source, a ground electrode provided on said second flow passage and proximate to said third flow passage, said ion source spraying said sample solution from a tip of said third flow passage and thereby ionizing it,

a gas supply passage provided around third flow passage, and

an analyzing unit for analyzing ions from said ion source.

5. The mass-analyzing apparatus for a living body fluid as claimed in claim 4, a portion of said third flow passage being formed of an electrically conductive material, said ion source ionizing said sample solution through an application of a voltage between said gas-supply passage and said third flow passage.

6. The mass-analyzing apparatus for a living body fluid as claimed in claim 4, wherein said first flow passage includes a microdialysis for collecting living body fluid from a living body as the sample to be analyzed by sending a solution having an osmotic pressure which is substantially similar to an osmotic pressure of the body fluid into said living body and obtaining said sample solution including said sample to be analyzed from said living body.

7. A living body fluid analyzing system comprising:

a microdialysis for sending a first solution having an osmotic pressure which is substantially similar to an osmotic pressure of a body fluid into a living body and extracting a second solution from said living body;

a first flow passage in which said second solution from said microdialysis flows;

a second flow passage which mixes said second solution with an organic solution;

a gas source;

a gas flow controller which controls a flow quantity of the gas from said gas source;

a third flow passage in which the gas introduced from said gas source flows;

an ion source having an orifice for spraying and ionizing said second solution from said second flow passage at an end of said third flow passage; and

a mass spectrometer for carrying out mass analysis of ions sprayed from said orifice.

8. A living body fluid analyzing system as claimed in claim 7, wherein said microdialysis includes a dialysis membrane.

9. A living body fluid analyzing system as claimed in claim 7, wherein said second flow passage includes a portion which is grounded.

10. A living body fluid analyzing system as claimed in claim 9, wherein said portion of said second flow passage includes an electrode.