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Methods for removing primer sequences and blocking restriction endonuclease recognition domains

  • US 6,190,889 B1
  • Filed: 01/07/1999
  • Issued: 02/20/2001
  • Est. Priority Date: 11/01/1996
  • Status: Expired due to Fees
First Claim
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1. A method for removing all or a part of a primer sequence from a primer extended product, comprising:

  • a) providing a primer sequence comprising a methylated portion of a restriction endonuclease recognition domain, wherein recognition of said domain by a restriction endonuclease requires at least one methylated nucleotide;

    b) performing template-directed primer extension using said primer and a nucleic acid segment to generate a primer extended product; and

    c) digesting said primer extended product with a restriction endonuclease that recognizes the resulting double-stranded restriction endonuclease recognition domain comprised by said primer sequence in said primer extended product.

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