Target-dependent reactions using structure-bridging oligonucleotides
First Claim
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1. A method, comprising:
- a) providing;
i) target nucleic acid comprising first and second non-contiguous single-stranded regions separated by an intervening region comprising a double-stranded region;
ii) a bridging oligonucleotide capable of binding to said first and second non-contiguous single-stranded regions;
iii) a second oligonucleotide capable of binding to a portion of said first non-contiguous single-stranded region; and
iii) a cleavage means;
b) mixing said target nucleic acid, said bridging oligonucleotide, said second oligonucleotide, and said cleavage means under conditions such that a cleavage structure is formed and either said second oligonucleotide or said bridging oligonucleotide is cleaved.
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Abstract
The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
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Citations
13 Claims
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1. A method, comprising:
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a) providing;
i) target nucleic acid comprising first and second non-contiguous single-stranded regions separated by an intervening region comprising a double-stranded region;
ii) a bridging oligonucleotide capable of binding to said first and second non-contiguous single-stranded regions;
iii) a second oligonucleotide capable of binding to a portion of said first non-contiguous single-stranded region; and
iii) a cleavage means;
b) mixing said target nucleic acid, said bridging oligonucleotide, said second oligonucleotide, and said cleavage means under conditions such that a cleavage structure is formed and either said second oligonucleotide or said bridging oligonucleotide is cleaved. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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