ACC synthase genes from pineapple

• US 6,194,639 B1
• Filed: 05/01/1997
• Issued: 02/27/2001
• Est. Priority Date: 05/01/1996
• Status: Expired due to Term

• Current Assignee: Golden Circle Limited (The Kraft Heinz Company), The State Of Queensland, The University of Queensland
• Original Assignee: Golden Circle Limited (The Kraft Heinz Company), The State Of Queensland, The University of Queensland
• Inventors: Sanewski, Garth, Botella, José
• Primary Examiner(s): Smith, Lynette R. F.
• Assistant Examiner(s): Nelson, Amy J.
• Application Number:
  US08/846,826
• Time in Patent Office: 1,398 Days
• Field of Search: 536/23.6, 536/23.2, 435/172.3, 435/320.1, 800/205, 800/283, 800/286, 800/298
• US Class Current: 800/298
• CPC Class Codes: C12N 15/8249 involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
• CPC Class Codes: C12N 15/8291 Hormone-influenced development
• CPC Class Codes: C12N 9/88 Lyases (4.)

Abstract

New ACC synthase genes from pineapple are disclosed which have utility as targets for the generation of transgenic plants in which the expression of ACC synthase is substantially controlled to effect the regulation of plant development, and, in particular, initiation of natural flowering.

Claims

1. An isolated nucleotide sequence encoding an ACC synthase enzyme comprising the sequence of amino acids identified by SEQ ID NO:

2.

2. The isolated nucleotide sequence of claim 1 wherein said isolated nucleotide sequence is identified by SEQ ID NO:

1.

13. A vector comprising at least one copy of the nucleotide sequence of claim 1 or claim 2.

15. The vector of claim 13 wherein said nucleotide sequence is under control of one or more regulatory nucleotide sequences.

19. A method of producing a transgenic pineapple plant, including the steps of introducing into a pineapple plant, plant part or plant cell a vector comprising the nucleotide sequence of claim 1 or claim 2 wherein said nucleotide sequence is operably linked, in the sense orientation, to one or more regulatory nucleotide sequences, and growing said plant, plant part or plant cell to produce the transgenic pineapple plant.

21. A method of producing a transgenic pineapple plant, including the steps of introducing into a pineapple plant, plant part or plant cell a vector comprising the nucleotide sequence of claim 1 or claim 2 wherein said nucleotide sequence is operably linked, in the antisense orientation, to one or more regulatory nucleotide sequences, and growing said plant, plant part or plant cell to produce the transgenic pineapple plant.

25. A transgenic pineapple plant transformed with a vector comprising the nucleotide sequence of claim 1 or claim 2 wherein said nucleotide sequence is operably linked, in the sense orientation, to one or more regulatory nucleotide sequences.

27. A transgenic pineapple plant transformed with a vector comprising the nucleotide sequence of claim 1 or claim 2 wherein said nucleotide sequence is operably linked, in the antisense orientation, to one or more regulatory nucleotide sequences.

31. A pineapple fruit, fruit part or fruit cell produced from the transgenic pineapple plant of claim 25.

33. A pineapple fruit, fruit part or fruit cell produced from the transgenic pineapple plant of claim 27.

3. An isolated nucleotide sequence encoding an ACC synthase enzyme comprising the sequence of amino acids identified by SEQ ID NO:

4.

4. The isolated nucleotide sequence of claim 3 wherein said isolated nucleotide sequence is identified by SEQ ID NO:

3.

14. A vector comprising at least one copy of the nucleotide sequence claim 3 or claim 4.

16. The vector of claim 14 wherein said nucleotide sequence is under control of one or more regulatory nucleotide sequences.

20. A method of producing a transgenic pineapple plant, including the steps of introducing into a pineapple plant, plant part or plant cell a vector comprising the nucleotide sequence of claim 3 or claim 4 wherein said nucleotide sequence is operably linked, in the sense orientation, to one or more regulatory nucleotide sequences, and growing said plant, plant part or plant cell to produce the transgenic pineapple plant.

22. A method of producing a transgenic pineapple plant, including the steps of introducing into a pineapple plant, plant part or plant cell a vector comprising the nucleotide sequence of claim 3 or claim 4 wherein said nucleotide sequence is operably linked, in the antisense orientation, to one or more regulatory nucleotide sequences, and growing said plant, plant part or plant cell to produce the transgenic pineapple plant.

26. A transgenic pineapple plant transformed with a comprising the nucleotide sequence of claim 3 or claim 4 wherein said nucleotide sequence is operably linked, in the sense orientation, to one or more regulatory nucleotide sequences.

28. A transgenic pineapple plant transformed with a vector comprising the nucleotide sequence of claim 3 for claim 4 wherein said nucleotide sequence is operably linked, in the antisense orientation, to one or more regulatory nucleotide sequences.

32. A pineapple fruit, fruit part or fruit cell produced from the transgenic pineapple plant of claim 26.

34. A pineapple fruit, fruit part or fruit cell produced from the transgenic pineapple plant of claim 28.

5. An isolated nucleotide sequence which is of sufficient length to regulate the level of ACC synthase gene expression and which hybridizes under high stringency wash conditions with SEQ ID NO:

1, wherein said high stringency wash conditions are selected from the group consisting of;

(i) 0.1×

SSC/0.1% SDS at about 68°

C. for at least about 20 minutes, and (ii) 0.2×

SSC/0.1% SDS at about 68°

C. for about one hour.

9. The isolated nucleotide sequence of any one of claims 5, 6, 7 and 8, wherein said isolated nucleotide sequence is obtained from a cell or tissue of a pineapple plant.

10. The isolated nucleotide sequence of any one of claims 5, 6, 7 and 8, wherein said isolated nucleotide sequence is obtained from a cell or tissue of a D leaf of a pineapple plant.

11. The isolated nucleotide sequence of any one of claims 5, 6, 7 and 8, wherein said isolated nucleotide sequence is obtained from a cell or tissue of a basal white portion of a D leaf of a pineapple plant.

12. The isolated nucleotide sequence of any one of claims 5, 6, 7 and 8, wherein said isolated nucleotide sequence is obtained from a cell or tissue of a stem apex of a pineapple plant.

17. A vector comprising at least one copy of the nucleotide sequence of any one of claims 5, 6, 7 and 8.

18. The vector of claim 17 wherein said nucleotide sequence is under control of one or more regulatory nucleotide sequences.

23. A method of producing a transgenic pineapple plant including the steps of introducing into a pineapple plant, plant part or plant cell a vector comprising the nucleotide sequence of any one of claims 5, 6, 7 and 8, wherein said nucleotide sequence is operably linked, in the sense orientation, to one or more regulatory nucleotide sequences, and growing said plant, plant part or plant cell to produce the transgenic pineapple plant.

24. A method of producing a transgenic pineapple plant, including the steps of introducing into a pineapple plant, plant part or plant cell a vector comprising the nucleotide sequence of any one of claims 5, 6, 7 and 8, wherein said nucleotide sequence is operably linked, in the antisense orientation, to one or more regulatory nucleotide sequences, and growing said plant, plant part or plant cell to produce the transgenic pineapple plant.

29. A transgenic pineapple plant transformed with a vector comprising the nucleotide sequence of any one of claims 5, 6, 7 and 8, wherein said nucleotide sequence is operably linked, in the sense orientation, to one or more regulatory nucleotide sequences.

30. A transgenic pineapple plant transformed with a vector comprising the nucleotide sequence of any one of claims 5, 6, 7 and 8, wherein said nucleotide sequence is operably linked, in the antisense orientation, to one or more regulatory nucleotide sequences.

35. A pineapple fruit, fruit part or fruit cell produced from the transgenic pineapple plant of claim 29.

36. A pineapple fruit, fruit part or fruit cell produced from the transgenic pineapple plant of claim 30.

6. An isolated nucleotide sequence which is of sufficient length to regulate the level of ACC synthase gene expression and which hybridizes under high stringency wash conditions with SEQ ID NO:

3, wherein said high stringency wash conditions are selected from the group consisting of;

(i) 0.1×

SSC/0.1% SDS at about 68°

C. for at least about 20 minutes, and (ii) 0.2×

SSC/0.1% SDS at about 68°

C. for about one hour.

7. An isolated ACC synthase nucleotide sequence which hybridizes under high stringency wash conditions with SEQ ID NO:

1, wherein said high stringency wash conditions are selected from the group consisting of;

(i) 0.1×

SSC/0.1% SDS at about 68°

C. for at least about 20 minutes, (ii) 0.2×

SSC/0.1% SDS at about 55°

C. for about one hour, (iii) 0.2×

SSC/0.1% SDS at about 62°

C. for about one hour, and (iv) 0.2×

SSC/0.1% SDS at about 68°

C. for about one hour.

8. An isolated ACC synthase nucleotide sequence which hybridizes under high stringency wash conditions with SEQ ID NO:

3, wherein said high stringency wash conditions are selected from the group consisting of;

(i) 0.1×

SSC/0.1% SDS at about 68°

C. for at least about 20 minutes, (ii) 0.2×

SSC/0.1% SDS at about 55°

C. for about one hour, (iii) 0.2×

SSC/0.1% SDS at about 62°

C. for about one hour, and (iv) 0.2×

SSC/0.1% SDS at about 68°

C. for about one hour.