Causative agent of the mystery swine disease, vaccine compositions and diagnostics kits

• US 6,197,310 B1
• Filed: 11/13/1996
• Issued: 03/06/2001
• Est. Priority Date: 06/06/1991
• Status: Expired due to Term

• Current Assignee: Stichting Dienst Landbouwkundig Onderzoek
• Original Assignee: Stichting Centraal Diergeneeskundig Instituut
• Inventors: Terpstra, Catharinus, Wensvoort, Gert, Pol, Joannes Maria Anthonis
• Primary Examiner(s): MOSHER, MARY
• Application Number:
  US08/747,863
• Time in Patent Office: 1,574 Days
• Field of Search: 424/204.1, 424/815, 435/235.1, 435/236, 435/237, 435/238
• US Class Current: 424/204.1
• CPC Class Codes: A61K 2039/525 Virus
• CPC Class Codes: A61K 2039/5252 inactivated (killed)
• CPC Class Codes: A61K 2039/5254 avirulent or attenuated
• CPC Class Codes: A61K 2039/53 DNA (RNA) vaccination
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 16/10 from RNA viruses
• CPC Class Codes: C12N 2770/10021 Viruses as such, e.g. new isolates, mutants or their genomic sequences
• CPC Class Codes: C12N 2770/10022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2770/10034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
• CPC Class Codes: C12N 7/00 Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 1/6888 for detection or identification of organisms
• CPC Class Codes: Y10S 424/815 Viral vaccine for porcine species, e.g. swine
• CPC Class Codes: Y10S 435/975 Kit

Abstract

Composition of matter comprising the causative agent of Mystery Swine Disease, Lelystad Agent, in a live, attenuated, dead, or recombinant form, or a part or component of it. Vaccine compositions and diagnostic kits based thereon. Recombinant nucleic acid comprising a Lelystad Agent-specific nucleotide sequence. Peptides comprising a Lelystad Agent-specific amino acid sequence. Lelystad Agent-specific antibodies.

Claims

1. A vaccine composition for vaccinating an animal, comprising:

a virus which is causative of Mystery Swine Disease and a suitable carrier, said virus characterized in being sensitive to chloroform and having a size smaller than 200 nanometers, said virus being specifically reactive with serum antibodies of a sow, said serum antibodies obtained by;

a) intranasally inoculating a specific pathogen free sow with two milliliters of the virus identified as deposit number I1102, deposited Jun. 5, 1991 with the Insitut Pasteur, Paris, France (at passage level 3, titer 10^4,8 TCID₅₀/milliliter); and

b) collecting serum antibodies from the thus inoculated sow after 25 to 33 days.

2. The vaccine composition of claim 1 further comprising an adjuvant.

3. The vaccine composition of claim 1 wherein said virus is unattenuated virus.

4. The vaccine composition of claim 1 wherein said virus is inactivated virus.

5. The vaccine composition of claim 4 further comprising an adjuvant.

6. The vaccine composition of claim 1 wherein said virus is attenuated virus serially passaged in pig lung macrophages.

7. A vaccine composition for vaccinating an animal, comprising:

an adjuvant; and

, admixed therewith,

a virus causative of Mystery Swine Disease, said virus specifically reactive with serum antibodies of a sow, said serum antibodies obtained by;

a) intranasally inoculating a specific pathogen free sow with two milliliters of the virus identified as deposit number I1102, deposited Jun. 5, 1991 with the Insitut Pasteur, Paris, France (at passage level 3, titer 10^4,8 TCID₅₀/milliliter); and

b) collecting serum antibodies from the thus inoculated sow after 25 to 33 days.