Materials and methods for making improved liposome compositions

• US 6,197,333 B1
• Filed: 12/18/1998
• Issued: 03/06/2001
• Est. Priority Date: 03/28/1996
• Status: Expired due to Term

• Current Assignee: Board of Trustees of The University of Illinois
• Original Assignee: Board of Trustees of The University of Illinois
• Inventors: Rubinstein, Israel, Onyuksel, Hayat
• Primary Examiner(s): Kishore, Gollamudi S.
• Application Number:
  US09/155,368
• Time in Patent Office: 809 Days
• Field of Search: 424/450, 424/1.21, 424/94.3, 424/9.321, 424/9.51, 424/417, 424/401, 428/402.2
• US Class Current: 424/450
• CPC Class Codes: A61K 38/2278 Vasoactive intestinal peptide [VIP]; Related peptides (e.g. Exendin)
• CPC Class Codes: A61K 49/0017 Fluorescence in vivo
• CPC Class Codes: A61K 49/0043 Fluorescein, used in vivo
• CPC Class Codes: A61K 49/0054 Macromolecular compounds, i.e. oligomers, polymers, dendrimers
• CPC Class Codes: A61K 49/0084 liposome, i.e. bilayered vesicular structure
• CPC Class Codes: A61K 49/1812 liposomes, polymersomes, e.g. immunoliposomes
• CPC Class Codes: A61K 49/227 Liposomes, lipoprotein vesicles, e.g. LDL or HDL lipoproteins, micelles, e.g. phospholipidic or polymeric
• CPC Class Codes: A61K 9/1271 Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers liposome as conjugate A61K47/6911
• CPC Class Codes: A61K 9/1278 Post-loading, e.g. by ion or pH gradient
• CPC Class Codes: A61P 17/02 for treating wounds, ulcers, burns, scars, keloids, or the like
• CPC Class Codes: A61P 39/06 Free radical scavengers or antioxidants

Abstract

Provided are methods for preparing improved biologically active liposome products comprising a biological1y active amphipathic compound in association with a liposome. Methods for producing the liposome products as well as methods of using the liposome products in therapcutic and diagnostic techniques are also provided.

Claims

1. A biologically active liposome product comprising a biologically active amphipathic compound in association with a liposome produced by a method comprising the steps of:

a) mixing a combination of lipids wherein said combination includes at least one lipid component covalently bonded to a water-soluble polymer;

b) forming sterically stabilized liposomes from said combination of lipids;

c) obtaining liposomes having an average diameter of less than about 300 nm; and

d) incubating liposomes from step (c) with a biologically active amphipathic compound under conditions in which said compound becomes associated with said liposomes from step (c) in an active conformation.

2. A composition comprising the biologically active liposome product of claim 1 wherein said biologically active amphipathic peptide has an activity selected from the group consisting of anti-oxidant activity, wound healing activity, anti-wrinkling activity, and anti-aging activity.

3. The composition according to claim 2 wherein the composition is a cosmetic.

4. The composition according to claim 2 wherein the composition is a therapeutic.

5. A diagnostic composition comprising the liposome composition according to claim 1 and further comprising a detectable label.

6. The diagnostic composition according to claim 5 wherein the label is selected from the group consisting of a fluorescent label, a radioactive label, a dye, and a compound which enhances magnetic resonance imaging.