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Compositions and methods for analysis of nucleic acids

  • US 6,197,557 B1
  • Filed: 09/10/1998
  • Issued: 03/06/2001
  • Est. Priority Date: 03/05/1997
  • Status: Expired due to Term
First Claim
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1. A method of preparing a DNA molecule comprising:

  • a) ligating an adaptor to the end of a template DNA molecule, wherein said ligation introduces a nick or gap site onto said template DNA molecule;

    b) initiating a nick translation reaction at the nick or gap site of said adaptor using a DNA polymerase having 5′

    to 3′

    exonuclease activity;

    c) allowing said nick translation reaction to proceed for a specific time to produce DNA fragments of similar size; and

    d) terminating said nick translation reaction.

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