Devices and methods for separating cellular components of blood from liquid portion of blood

• US 6,197,598 B1
• Filed: 08/19/1997
• Issued: 03/06/2001
• Est. Priority Date: 05/09/1995
• Status: Expired due to Fees

• Current Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Original Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Inventors: Jaseph, Corey L., Schrier, Wayne H., Ruggeri, Franco, Schoengold, Ronald J.
• Primary Examiner(s): Chin, Christopher L.
• Assistant Examiner(s): Do, Pensee T.
• Application Number:
  US08/914,502
• Time in Patent Office: 1,295 Days
• Field of Search: 422/56, 422/58, 422/101, 435/2, 435/4, 435/7.1, 435/7.92, 435/287.1, 435/287.2, 435/287.7, 435/287.8, 435/287.9, 435/805, 435/810, 435/962, 435/970, 436/514, 436/518, 436/169, 436/170, 436/177, 436/178, 436/808, 436/810
• US Class Current: 436/518
• CPC Class Codes: B01D 15/3804 Affinity chromatography
• CPC Class Codes: G01N 2333/205 from Campylobacter (G)
• CPC Class Codes: G01N 30/02 Column chromatography
• CPC Class Codes: G01N 30/90 Plate chromatography, e.g. thin layer or paper chromatography
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: G01N 33/5002 Partitioning blood components
• CPC Class Codes: G01N 33/521 Single-layer analytical elements
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/54386 Analytical elements
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 435/81 Packaged device or kit
• CPC Class Codes: Y10S 435/962 Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 436/808 Automated or kit
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A device for the separation of the liquid portion of blood from the cellular components of blood comprises: (1) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood; (2) a substrate supporting the pad; and (3) means, attached to the pad, for facilitating the flow of the liquid portion of the blood: (i) through interstices around the trapped cellular components of the blood and (ii) from the pad of porous material. The separation of the liquid portion of blood from the cellular components of the blood occurs by flow through the pad of porous material without significant hemolysis. The device can be incorporated into a device for the performance of specific binding assays such as immunoassays. The pad of porous material can contain an agglutinating agent such as a lectin or an anti-blood cell antibody, or a carbohydrate such as mannitol. Other devices and methods for the separation of the liquid portion of blood from the cellular components of the blood are also disclosed.

Claims

1. A method for separating the liquid portion of blood from the cellular components of blood comprising the steps of:

(a) adding a cross-linking substance for the cellular components of blood to a sample of whole blood, the cross-linking substance being selected from the group consisting of a lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;

(b) mixing the cross-linking substance and the blood sample to form a mixture of the cross-linking substance and the blood sample;

(c) applying the mixture of the cross-linking substance and the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device comprising;

(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;

(ii) a substrate supporting the pad; and

(iii) means, attached to the pad, for facilitating the flow of the liquid portion of the blood;

(1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of blood from the cellular components of the blood occurs by flow through the pad without significant hemolysis; and

(d) allowing the liquid portion of the blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood.

2. The method of claim 1 further comprising adding an anticoagulant together with the cross-linking substance.

3. The method of claim 2 wherein the anticoagulant is selected from the group consisting of EDTA and heparin.

4. The method of claim 1 wherein a concentration of cross-linking substance is used that is sufficient to cross-link substantially all cellular elements of blood.

5. A method for separating the liquid portion of blood from the cellular components of blood comprising the steps of:

(a) adding a sample of blood to a capillary tube coating with a cross-linking substance, the cross-linking substance being selected from the group consisting of a lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;

(b) allowing the cross-linking substance to dissolve in the blood sample to form a mixture of the cross-linking substance and the blood sample;

(c) applying the mixture of the cross-linking substance and the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device comprising;

(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;

(ii) a substrate supporting the pad; and

(iii) means, attached to the pad, for facilitating the flow of the liquid portion of the blood (1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of blood from the cellular components of the blood occurs by flow through the pad without significant hemolysis; and

(d) allowing the liquid portion of the blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood.

6. The method of claim 5 wherein the capillary tube is also coated with an anticoagulant together with the cross-linking substance and the anticoagulant is allowed to dissolve in the blood sample.

7. The method of claim 6 wherein the anticoagulant is selected from the group consisting of EDTA and heparin.

8. The method of claim 5 wherein a concentration of cross-linking substance is used that is sufficient to cross-link substantially all cellular elements of blood.

9. A method for detecting or determining an analyte in a liquid portion of blood where the liquid portion of blood is separated from the cellular components of blood comprising the steps of:

(a) adding a cross-linking substance for the cellular components of blood to a sample of whole blood, the cross-linking substance being selected from the group consisting of a lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;

(b) mixing the cross-linking substance and the blood sample to form a mixture of the cross-linking substance and the blood sample;

(c) applying the mixture of the cross-linking substance and the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device comprising;

(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;

(ii) a substrate supporting the pad; and

(iii) means, attached to the pad for facilitating the flow of the liquid portion of the blood;

(1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of the blood from the cellular components of blood occurs by flow through the pad without significant hemolysis;

(d) allowing the liquid portion of blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood; and

(e) applying the liquid portion of the blood to a chromatographic medium to perform an assay for the analyte in the chromatographic medium to detect or determine the analyte.

10. The method of claim 9 further comprising adding an anticoagulant together with the cross-linking substance.

11. The method of claim 10 wherein the anticoagulant is selected from the group consisting of EDTA and heparin.

12. The method of claim 9 wherein a concentration of cross-linking substance is used that is sufficient to cross-link substantially all cellular elements of blood.

13. The method of claim 9 wherein the step of performing the assay for the analyte on the chromatographic medium involves a mobile labeled specific binding partner for the analyte and an immobilized specific binding partner for the analyte on the chromatographic medium so that a ternary complex forms in the chromatographic medium at the location of the immobilized specific binding partner for the analyte if analyte is present in the sample.

14. The method of claim 13 wherein the mobile labeled specific binding partner is labeled with a visually detectable label.

15. A method for detecting or determining an analyte in a liquid portion of blood where the liquid portion of blood is separated from the cellular components of blood comprising the steps of:

(a) adding a sample of blood to a capillary tube coated with a cross-linking substance, the cross-linking substance being selected from the group consisting of a lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;

(b) allowing the cross-linking substance to dissolve in the blood sample to form a mixture of the cross-linking substance and the blood sample;

(c) applying the mixture of a cross-linking substance and the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device comprising;

(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;

(ii) a substrate supporting the pad; and

(iii) means, attached to the pad, for facilitating the flow of the liquid portion of the blood;

(1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of blood from the cellular components of blood occurs by flow through the pad without significant hemolysis;

(d) allowing the liquid portion of the blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood; and

(e) applying the liquid portion of the blood to a chromatographic medium to perform an assay for the analyte in the chromatographic medium to detect or determine the analyte.

16. The method of claim 15 wherein the capillary tube is also coated with an anticoagulant together with the cross-linking substance and the anticoagulant is allowed to dissolve in the blood sample.

17. The method of claim 16 wherein the anticoagulant is selected from the group consisting of EDTA and heparin.

18. The method of claim 15 wherein a concentration of cross-linking substance is used that is sufficient to cross-link substantially all cellular elements of blood.

19. The method of claim 15 wherein the step of performing the assay for the analyte involves a mobile labeled specific binding partner for the analyte and an immobilized specific binding partner for the analyte on the chromatographic medium so that a ternary complex forms in the chromatographic medium at the location of the immobilized specific binding partner for the analyte if analyte is present in the sample.

20. The method of claim 19 wherein the labeled specific binding partner for the analyte is labeled with a visibly detectable label.

21. The method of claim 19 further consisting essentially of adding an anticoagulant together with the cross-linking substance.

22. The method of claim 21 wherein the anticoagulant is selected from the group consisting of EDTA and heparin.

23. A method for separating the liquid portion of blood from the cellular components of blood consisting essentially of the steps of:

(a) adding a cross-linking substance for the cellular components of blood to a sample of whole blood, the cross-linking substance being selected from the group consisting of lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;

(b) mixing the cross-linking substance in the blood sample to form a mixture consisting essentially of the cross-linking substance and the blood sample;

(c) applying the mixture of the crosslinking substance in the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device consisting essentially of;

(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;

(ii) a substrate supporting the pad; and

(iii) means, attached to the pad, for facilitating the flow of the liquid portion of the blood;

(1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of blood from the cellular components of blood occurs by flow through the pad without significant hemolysis;

(d) allowing the liquid portion of the blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood.

24. The method of claim 23 wherein a concentration of cross-linking substance is used that is sufficient to cross-link substantially all cellular elements of blood.

25. A method for separating the liquid portion of blood from the cellular components of blood consisting essentially of the steps of:

(a) adding a sample of blood to a capillary tube coated with a cross-linking substance, the cross-linking substance being selected from the group consisting of a lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;

(b) allowing the cross-linking substance to dissolve in the blood sample to form a mixture consisting essentially of the cross-linking substance and the blood sample;

(c) applying the mixture of the cross-linking substance in the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device consisting essentially of;

(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;

(ii) a substrate supporting the pad; and

(iii) means, attached to the pad, for facilitating the flow of the liquid portion of the blood;

(1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of blood from the cellular components of the blood occurs by flow through the pad without significant hemolysis; and

(d) allowing the liquid portion of the blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood.

26. The method of claim 25 wherein the capillary tube is also coated with an anticoagulant together with the cross-linking substance and the anticoagulant is allowed to dissolve in the blood sample.

27. The method of claim 26 wherein the anticoagulant is selected from the group consisting of EDTA and heparin.

28. The method of claim 25 wherein a concentration of cross-linking substances used that is sufficient to cross-link substantially all cellular elements of blood.