Devices and methods for separating cellular components of blood from liquid portion of blood
First Claim
1. A method for separating the liquid portion of blood from the cellular components of blood comprising the steps of:
- (a) adding a cross-linking substance for the cellular components of blood to a sample of whole blood, the cross-linking substance being selected from the group consisting of a lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;
(b) mixing the cross-linking substance and the blood sample to form a mixture of the cross-linking substance and the blood sample;
(c) applying the mixture of the cross-linking substance and the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device comprising;
(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;
(ii) a substrate supporting the pad; and
(iii) means, attached to the pad, for facilitating the flow of the liquid portion of the blood;
(1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of blood from the cellular components of the blood occurs by flow through the pad without significant hemolysis; and
(d) allowing the liquid portion of the blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood.
1 Assignment
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Accused Products
Abstract
A device for the separation of the liquid portion of blood from the cellular components of blood comprises: (1) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood; (2) a substrate supporting the pad; and (3) means, attached to the pad, for facilitating the flow of the liquid portion of the blood: (i) through interstices around the trapped cellular components of the blood and (ii) from the pad of porous material. The separation of the liquid portion of blood from the cellular components of the blood occurs by flow through the pad of porous material without significant hemolysis. The device can be incorporated into a device for the performance of specific binding assays such as immunoassays. The pad of porous material can contain an agglutinating agent such as a lectin or an anti-blood cell antibody, or a carbohydrate such as mannitol. Other devices and methods for the separation of the liquid portion of blood from the cellular components of the blood are also disclosed.
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Citations
28 Claims
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1. A method for separating the liquid portion of blood from the cellular components of blood comprising the steps of:
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(a) adding a cross-linking substance for the cellular components of blood to a sample of whole blood, the cross-linking substance being selected from the group consisting of a lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;
(b) mixing the cross-linking substance and the blood sample to form a mixture of the cross-linking substance and the blood sample;
(c) applying the mixture of the cross-linking substance and the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device comprising;
(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;
(ii) a substrate supporting the pad; and
(iii) means, attached to the pad, for facilitating the flow of the liquid portion of the blood;
(1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of blood from the cellular components of the blood occurs by flow through the pad without significant hemolysis; and
(d) allowing the liquid portion of the blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood. - View Dependent Claims (2, 3, 4)
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5. A method for separating the liquid portion of blood from the cellular components of blood comprising the steps of:
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(a) adding a sample of blood to a capillary tube coating with a cross-linking substance, the cross-linking substance being selected from the group consisting of a lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;
(b) allowing the cross-linking substance to dissolve in the blood sample to form a mixture of the cross-linking substance and the blood sample;
(c) applying the mixture of the cross-linking substance and the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device comprising;
(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;
(ii) a substrate supporting the pad; and
(iii) means, attached to the pad, for facilitating the flow of the liquid portion of the blood (1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of blood from the cellular components of the blood occurs by flow through the pad without significant hemolysis; and
(d) allowing the liquid portion of the blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood. - View Dependent Claims (6, 7, 8)
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9. A method for detecting or determining an analyte in a liquid portion of blood where the liquid portion of blood is separated from the cellular components of blood comprising the steps of:
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(a) adding a cross-linking substance for the cellular components of blood to a sample of whole blood, the cross-linking substance being selected from the group consisting of a lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;
(b) mixing the cross-linking substance and the blood sample to form a mixture of the cross-linking substance and the blood sample;
(c) applying the mixture of the cross-linking substance and the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device comprising;
(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;
(ii) a substrate supporting the pad; and
(iii) means, attached to the pad for facilitating the flow of the liquid portion of the blood;
(1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of the blood from the cellular components of blood occurs by flow through the pad without significant hemolysis;
(d) allowing the liquid portion of blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood; and
(e) applying the liquid portion of the blood to a chromatographic medium to perform an assay for the analyte in the chromatographic medium to detect or determine the analyte. - View Dependent Claims (10, 11, 12, 13, 14)
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15. A method for detecting or determining an analyte in a liquid portion of blood where the liquid portion of blood is separated from the cellular components of blood comprising the steps of:
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(a) adding a sample of blood to a capillary tube coated with a cross-linking substance, the cross-linking substance being selected from the group consisting of a lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;
(b) allowing the cross-linking substance to dissolve in the blood sample to form a mixture of the cross-linking substance and the blood sample;
(c) applying the mixture of a cross-linking substance and the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device comprising;
(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;
(ii) a substrate supporting the pad; and
(iii) means, attached to the pad, for facilitating the flow of the liquid portion of the blood;
(1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of blood from the cellular components of blood occurs by flow through the pad without significant hemolysis;
(d) allowing the liquid portion of the blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood; and
(e) applying the liquid portion of the blood to a chromatographic medium to perform an assay for the analyte in the chromatographic medium to detect or determine the analyte. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22)
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23. A method for separating the liquid portion of blood from the cellular components of blood consisting essentially of the steps of:
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(a) adding a cross-linking substance for the cellular components of blood to a sample of whole blood, the cross-linking substance being selected from the group consisting of lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;
(b) mixing the cross-linking substance in the blood sample to form a mixture consisting essentially of the cross-linking substance and the blood sample;
(c) applying the mixture of the crosslinking substance in the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device consisting essentially of;
(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;
(ii) a substrate supporting the pad; and
(iii) means, attached to the pad, for facilitating the flow of the liquid portion of the blood;
(1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of blood from the cellular components of blood occurs by flow through the pad without significant hemolysis;
(d) allowing the liquid portion of the blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood. - View Dependent Claims (24)
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25. A method for separating the liquid portion of blood from the cellular components of blood consisting essentially of the steps of:
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(a) adding a sample of blood to a capillary tube coated with a cross-linking substance, the cross-linking substance being selected from the group consisting of a lectin, an anti-blood cell antibody, and a carbohydrate capable of agglutinating blood cells;
(b) allowing the cross-linking substance to dissolve in the blood sample to form a mixture consisting essentially of the cross-linking substance and the blood sample;
(c) applying the mixture of the cross-linking substance in the blood sample to a device for separating the liquid portion of blood from the cellular components of blood, the device consisting essentially of;
(i) a pad of porous material permeable to the liquid portion of blood but capable of trapping the cellular components of blood aggregated by the reaction between the cross-linking substance and the blood sample;
(ii) a substrate supporting the pad; and
(iii) means, attached to the pad, for facilitating the flow of the liquid portion of the blood;
(1) through interstices around the trapped cellular components of the blood and (2) from the pad of porous material, whereby the separation of the liquid portion of blood from the cellular components of the blood occurs by flow through the pad without significant hemolysis; and
(d) allowing the liquid portion of the blood to flow through the pad to separate the liquid portion of the blood from the cellular components of the blood. - View Dependent Claims (26, 27, 28)
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Specification