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Fluorescent protein sensors for detection of analytes

  • US 6,197,928 B1
  • Filed: 03/14/1997
  • Issued: 03/06/2001
  • Est. Priority Date: 03/14/1997
  • Status: Expired due to Term
First Claim
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1. A fluorescent indicator protein comprising:

  • a binding protein moiety having an analyte-binding domain which binds an analyte and causes the indicator to change conformation upon exposure to the analyte;

    a donor fluorescent protein moiety covalently coupled to the binding protein moiety; and

    an acceptor fluorescent protein moiety covalently coupled to the binding protein moiety, wherein a conformational change is induced in the binding protein moiety upon analyte binding, which in turn induces conformational changes in the position or orientation of the donor fluorescent protein and acceptor fluorescent protein moieties with respect to one another, thereby altering the relative amounts of fluorescence from the two fluorescent protein moieties when the donor is excited by irradiation.

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