Insulin promoter factor, and uses related thereto

• US 6,197,945 B1
• Filed: 02/27/1998
• Issued: 03/06/2001
• Est. Priority Date: 10/07/1994
• Status: Expired due to Fees

• Current Assignee: ES Cell International Pte Ltd. (Lineage Cell Therapeutics, Inc.)
• Original Assignee: Ontogeny, Inc. (Curis, Inc.)
• Inventors: Edlund, Thomas
• Primary Examiner(s): Zitomer, Stephanie W.
• Assistant Examiner(s): WILDER, CYNTHIA B
• Application Number:
  US09/031,898
• Time in Patent Office: 1,103 Days
• Field of Search: 435/320.1, 435/91.2, 435/6, 435/325, 536/23.1, 536/24.3, 536/24.33, 536/24.1, 530/350
• US Class Current: 536/23.1
• CPC Class Codes: A01K 2217/05 Animals comprising random inserted nucleic acids (transgenic)
• CPC Class Codes: A01K 2217/075 inducing loss of function, i.e. knock out
• CPC Class Codes: A01K 2227/105 Murine
• CPC Class Codes: A01K 2267/03 Animal model, e.g. for test or diseases
• CPC Class Codes: A01K 2267/0325 Animal model for autoimmune diseases
• CPC Class Codes: A01K 2267/0331 Animal model for proliferative diseases
• CPC Class Codes: A01K 67/0276 Knock-out vertebrates
• CPC Class Codes: C07K 14/4702 Regulators; Modulating activity
• CPC Class Codes: C07K 14/4705 stimulating, promoting or activating activity
• CPC Class Codes: C12N 15/8509 for producing genetically modified animals, e.g. transgenic
• CPC Class Codes: C12N 2800/30 Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Abstract

The present invention relates to the discovery in eukaryotic cells, particularly mammalian cells, of novel a transcriptional regulatory factor, referred to hereinafter as “Insulin Promoter Factor 1” or “Ipf1”.

Claims

1. An isolated or recombinant nucleic acid encoding a recombinant insulin promoter fator 1 (Ipf1) polypeptide comprising a polypeptide sequence at least 90% identical to an amino acid sequence represented in SEQ ID NO:

2, wherein said polypetide modulates at least one of proliferation, differentiation or survival of a cell which expresses a gene that is transcriptionally regulated by an Ipf1-responsive element (Ipf1-RE).

2. The nucleic acid of claim 1, wherein said nucleotide sequence hybridizes under conditions including a wash step of 0.2×

SSC at 50°

C. to a nucleic acid probe having a sequence represented by at least 12 consecutive nucleotides of SEQ ID NO;

1.

3. The nucleic acid of claim 1, wherein said nucleotide sequence hybridizes under conditions including a wash step of 0.2×

SSC at 50°

C. to a nucleic acid probe having a sequence represented by at least 12 consecutive nucleotides between nucleotide residues 31 to 562 or 761 to 979 of SEQ ID NO;

1.

4. The nucleic acid of claim 1, further comprising a transcriptional regulatory sequence operably linked to said nucleotide sequence so as to render said nucleic acid suitable for use as an expression vector.

5. An expression vector, capable of replicating in at least one of a prokaryotic cell and eukaryotic cell, comprising the nucleic acid of claim 1.

6. A host cell transfected with the expression vector of claim 5 expressing said recombinant polypeptide.

7. The nucleic acid of claim 1, wherein the Ipf1 polypeptide sequence is at least 95% identical to an amino acid sequence represented in SEQ ID NO:

2.