Grapevine leafroll virus (type 2) proteins and their uses

• US 6,197,948 B1
• Filed: 05/19/1998
• Issued: 03/06/2001
• Est. Priority Date: 05/20/1997
• Status: Expired due to Fees

• Current Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Original Assignee: Cornell Research Foundation Incorporated (Cornell University)
• Inventors: Zhu, Hai-Ying, Gonsalves, Dennis, Ling, Kai-Shu
• Primary Examiner(s): Fox, David T.
• Assistant Examiner(s): Mehta, Ashwin D.
• Application Number:
  US09/080,983
• Time in Patent Office: 1,022 Days
• Field of Search: 435/69.1, 435/320.1, 435/410, 435/414, 435/419, 435/468, 435/469, 435/470, 536/23.72, 800/278, 800/279, 800/280, 800/288, 800/293, 800/294, 800/295, 800/301, 800/317.3
• US Class Current: 536/23.72
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C12N 15/8283 for virus resistance
• CPC Class Codes: C12N 2770/00022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12Q 1/70 involving virus or bacteriophage immunoassay for viruses G01N33/56983
• CPC Class Codes: G01N 2333/08 RNA viruses
• CPC Class Codes: G01N 33/56961 Plant cells or fungi
• CPC Class Codes: G01N 33/56983 Viruses

Abstract

The present invention relates to isolated proteins or polypeptides of grapevine leafroll virus (type 2). The encoding DNA molecules either alone in isolated form or in an expression system, a host cell, or a transgenic grape plant are also disclosed. Other aspects of the present invention relates to a method of imparting grapevine leafroll resistance to grape and tobacco plants by transforming them with the DNA molecules of the present invention, a method of imparting beet yellows virus resistance to a beet plant, a method of imparting tristeza virus resistance to a citrus plant, and a method of detecting the presence of a grapevine leafroll virus, such as GRLaV-2, in a sample.

Claims

1. An isolated RNA molecule encoding a protein or polypeptide of grapevine leafroll virus (type 2) wherein the protein or polypeptide is selected from a group consisting of a heat shock 70 protein and a coat protein.

2. An isolated DNA molecule encoding a protein or polypeptide of a grapevine leafroll virus (type 2) wherein the protein or polypeptide is a heat shock 70 protein having a molecular weight of from about 63 to about 67 kDa.

3. An isolated DNA molecule according to claim 2, wherein the protein or polypeptide comprises an amino acid sequence corresponding to SEQ. ID. No. 9.

4. An isolated DNA molecule according to claim 3, wherein the DNA molecule has a nucleotide sequence corresponding to SEQ. ID. No. 8.

5. An isolated DNA molecule encoding a coat protein from grapevine leafroll virus (type 2) having a molecular weight of from about 20 to about 24 kDa.

6. An isolated DNA molecule according to claim 5 wherein the coat protein comprises an amino acid sequence corresponding to SEQ. ID. No. 15.

7. An isolated DNA molecule encoding a protein or polypeptide of grapevine leafroll virus (type 2), wherein the DNA molecule has a nucleotide sequence corresponding to SEQ. ID. No. 14.