Cation-selective sensor

• US 6,200,444 B1
• Filed: 11/25/1998
• Issued: 03/13/2001
• Est. Priority Date: 03/29/1996
• Status: Expired due to Fees

• Current Assignee: Institut FUER Chemo und Biosensorik Muenster EV
• Original Assignee: Institut FUER Chemo und Biosensorik Muenster EV
• Inventors: Ahlers, Benedikt, Cammann, Karl, Choulga, Alexandre
• Primary Examiner(s): Tung, T.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/155,510
• Time in Patent Office: 839 Days
• Field of Search: 422/82.01, 422/82.02, 204/417, 204/418, 204/419, 204/416, 324/438, 324/439
• US Class Current: 204/418
• CPC Class Codes: G01N 27/126 comprising organic polymers
• CPC Class Codes: G01N 31/221 for investigating pH value

Abstract

The invention concerns a cation-selective sensor provided with a cation-selective coating and based on the fact that analyte ions present in a solution cause detectable changes in the electrical characteristics of the layer. The acid/base components in the cation-selective layer render the sensor function independent of the anions present in the analyte solution. This improves the measurement accuracy and lowers the detection threshold.

Claims

1. Cation-selective sensor for the qualitative and/or quantitative determination of cations in solutions, characterized in that

the cation-selective sensor contains at least one ionically conducting cation-specific layer which is in contact with the solution and is made of a liquid, solid or semi-solid material,

in that this layer contains coupling elements which selectively remove the cations from the solution, so that the resistance, conductance, admittance or impedance of the layer is changed by cation uptake, in that the cation-specific layer contains at least one acid/base component, and in that the sensor comprises at least two electrodes, at least one of which is connected to the cation-specific layer.

2. Cation-selective sensor according to claim 1, characterized in that the acid/base component is present in the cation-specific layer in the form of functional groups with acid/base properties.

3. Cation-selective sensor according to claim 1, characterized in that the acid/base component is present in the cation-specific layer in the form of at least one chemical compound with acid/base properties.

4. Cation-selective sensor according to claim 1, characterized in that the cation-specific layer contains a component to which the acid/base component is bound.

5. Cation-selective sensor according to claim 1, characterized in that the acid/base component is lipophilic.

6. Cation-selective sensor according to claim 1, characterized in that the acid/base component is lipophilic acid or lipophilic base.

7. Cation-selective sensor according to claim 1, characterized in that the acid/base component has an acid dissociation constant pK_a in the range from 3 to 12.

8. Cation-selective sensor according to claim 1, characterized in that the acid/base component has an acid dissociation constant pK_a in the range from 6 to 8.

9. Cation-selective sensor according to claim 1, characterized in that the acid/base component is a pH indicator or a derivative of a pH indicator.

10. Cation-selective sensor according to claim 9, characterized in that the pH indicator or the derivative is lipophilic.

11. Cation-selective sensor according to claim 9, characterized in that the pH indicator or a derivative is lipophilically modified by the coupling of benzyl radicals or alkane chains.

12. Cation-selective sensor according to claim 9, characterized in that the acid/base component is a phenolic compound such as p-nitrophenol, a compound or a derivative of fluorescein, of a fluorescein ester, of 7-hydroxycoumarin, of resorufin, of flavone or of pyren-3-ol, or a component such as 7-(n-decyl)-2-methyl-4-(3′

,5′

-dichlorophen-4′

-one)indonaphthol.

13. Cation-selective sensor according to claim 1, characterized in that the acid/base component is fatty acid, a lipid or a surfactant.

14. Cation-selective sensor according to claim 1, characterized in that the acid/base component is 3-hydroxy-4-(4-nitrophenolazo)phenylocatadecanoate.

15. Cation-selective sensor according to claim 1, characterized in that the cation-specific layer contains at least one of the components:

organic liquid, plasticizer, ion exchanger, polymer, support material or an arbitrary combination of these components.

16. Cation-selective sensor according to claim 1, characterized in that the cation-specific layer is lipophilic.

17. Cation-selective sensor according to claim 1, characterized in that the cation-specific layer has a thickness of between 0.01 μ

m and 1 mm.

18. Cation-selective sensor according to claim 1, characterized in that the cation-specific layer contains 20-80% by weight of polymer material, 20-80% by weight of plasticizer, 1-10% by weight of cation-selective coupling elements and 1-10% by weight of acid/base components.

19. Cation-selective sensor according to claim 18, characterized in that the cation-specific layer contains 1-10% by weight of ion exchanger.

20. Cation-selective sensor according to claim 1, characterized in that the cation-specific layer is applied to an inert support.

21. Cation-selective sensor according to claim 1, characterized in that a further layer is applied to the cation-specific layer.

22. Cation-selective sensor according to claim 21, characterized in that the further layer contains at least one soluble buffer.

23. Cation-selective sensor according to claim 21, characterized in that the further layer contains at least one component used for preparation of the cation solution to be analyzed.

24. Cation-selective sensor according to claim 21, characterized in that the further layer contains a porous support or matrix stabilizing the cation-specific layer.

25. Cation-selective sensor according to claim 21, characterized in that the individual components of the further layer are soluble in the solution and adjust the pH and/or ionic strength of the solution.

26. Cation-selective sensor according to claim 21, characterized in that the further layer has a thickness of between 0.01 μ

m and 1 mm.

27. Cation-selective sensor according to claim 1, characterized in that the sensor is designed as a test strip.