Methods and reagents for quantitation of HLA-DR expression on peripheral blood cells
First Claim
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1. A composition for flow cytometric measurement of HLA-DR on human peripheral blood cells, comprising:
- a fluorophore-conjugated anti-HLA-DR antibody, and a lysosomotropic amine.
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Abstract
Improved methods, reagents, and kits for quantitation of HLA-DR expression on peripheral blood cells, particularly peripheral blood monocytes, are presented. Inclusion of a lysosomotropic amine, such as chloroquine, during staining stabilizes HLA-DR expression, and use of a novel anti-CD14 conjugate, anti-CD14-PerCP/CY5.5, permits the ready discrimination of monocytes. The improved methods, reagents, and kits may be used to assess immune competence, and to direct and monitor immunostimulatory therapies in septic patients exhibiting monocyte deactivation.
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9 Claims
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1. A composition for flow cytometric measurement of HLA-DR on human peripheral blood cells, comprising:
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a fluorophore-conjugated anti-HLA-DR antibody, and a lysosomotropic amine. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
a composition according to claim 1; and
an erythrocyte lysing composition.
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9. The kit according to claim 8, further comprising:
- pelletized beads conjugated with defined levels of PE.
Specification